title geo_accession status submission_date last_update_date type channel_count source_name_ch1 organism_ch1 characteristics_ch1 characteristics_ch1.1 characteristics_ch1.2 characteristics_ch1.3 growth_protocol_ch1 molecule_ch1 extract_protocol_ch1 label_ch1 label_protocol_ch1 taxid_ch1 hyb_protocol scan_protocol description description.1 data_processing platform_id contact_name contact_email contact_institute contact_address contact_city contact_state contact_zip/postal_code contact_country supplementary_file data_row_count culture type:ch1 genotype/variation:ch1 treatment duration:ch1 treatment:ch1 Pseudomonas syringae pv. tomato DC3000 Wild type Dark Rep1 GSM2715489 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min Dark Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Darkness treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G0 Gene expression after 10 min darkness treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715489/suppl/GSM2715489_Dark_Rep_1_Raw.txt.gz 15744 Liquid Wild type 10 min Darkness Pseudomonas syringae pv. tomato DC3000 Wild type Dark Rep2 GSM2715490 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min Dark Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Darkness treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G0 Gene expression after 10 min darkness treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715490/suppl/GSM2715490_Dark_Rep_2_Raw.txt.gz 15744 Liquid Wild type 10 min Darkness Pseudomonas syringae pv. tomato DC3000 Wild type Dark Rep3 GSM2715491 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min Dark Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Darkness treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G0 Gene expression after 10 min darkness treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715491/suppl/GSM2715491_Dark_Rep_3_Raw.txt.gz 15744 Liquid Wild type 10 min Darkness Pseudomonas syringae pv. tomato DC3000 Wild type Dark Rep4 GSM2715492 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min Dark Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Darkness treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G0 Gene expression after 10 min darkness treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715492/suppl/GSM2715492_Dark_Rep_4_Raw.txt.gz 15744 Liquid Wild type 10 min Darkness Pseudomonas syringae pv. tomato DC3000 Wild type Blue Rep 1 GSM2715493 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Blue light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Blue light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G1 Gene expression after 10 min blue light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715493/suppl/GSM2715493_Blue_Rep_1_Raw.txt.gz 15744 Liquid Wild type 10 min Blue light Pseudomonas syringae pv. tomato DC3000 Wild type Blue Rep 2 GSM2715494 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Blue light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Blue light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G1 Gene expression after 10 min blue light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715494/suppl/GSM2715494_Blue_Rep_2_Raw.txt.gz 15744 Liquid Wild type 10 min Blue light Pseudomonas syringae pv. tomato DC3000 Wild type Blue Rep 3 GSM2715495 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Blue light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Blue light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G1 Gene expression after 10 min blue light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715495/suppl/GSM2715495_Blue_Rep_3_Raw.txt.gz 15744 Liquid Wild type 10 min Blue light Pseudomonas syringae pv. tomato DC3000 Wild type Blue Rep 4 GSM2715496 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Blue light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Blue light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G1 Gene expression after 10 min blue light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715496/suppl/GSM2715496_Blue_Rep_4_Raw.txt.gz 15744 Liquid Wild type 10 min Blue light Pseudomonas syringae pv. tomato DC3000 Wild type Red Rep 1 GSM2715497 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Red light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Red light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G2 Gene expression after 10 min red light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715497/suppl/GSM2715497_Red_Rep_1_Raw.txt.gz 15744 Liquid Wild type 10 min Red light Pseudomonas syringae pv. tomato DC3000 Wild type Red Rep 2 GSM2715498 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Red light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Red light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G2 Gene expression after 10 min red light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715498/suppl/GSM2715498_Red_Rep_2_Raw.txt.gz 15744 Liquid Wild type 10 min Red light Pseudomonas syringae pv. tomato DC3000 Wild type Red Rep 3 GSM2715499 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Red light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Red light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G2 Gene expression after 10 min red light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715499/suppl/GSM2715499_Red_Rep_3_Raw.txt.gz 15744 Liquid Wild type 10 min Red light Pseudomonas syringae pv. tomato DC3000 Wild type Red Rep 4 GSM2715500 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under Red light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: Red light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G2 Gene expression after 10 min red light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715500/suppl/GSM2715500_Red_Rep_4_Raw.txt.gz 15744 Liquid Wild type 10 min Red light Pseudomonas syringae pv. tomato DC3000 Wild type White Rep 1 GSM2715501 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under White light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: White light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G3 Gene expression after 10 min white light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715501/suppl/GSM2715501_White_Rep_1_Raw.txt.gz 15744 Liquid Wild type 10 min White light Pseudomonas syringae pv. tomato DC3000 Wild type White Rep 2 GSM2715502 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under White light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: White light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G3 Gene expression after 10 min white light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715502/suppl/GSM2715502_White_Rep_2_Raw.txt.gz 15744 Liquid Wild type 10 min White light Pseudomonas syringae pv. tomato DC3000 Wild type White Rep 3 GSM2715503 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under White light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: White light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G3 Gene expression after 10 min white light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715503/suppl/GSM2715503_White_Rep_3_Raw.txt.gz 15744 Liquid Wild type 10 min White light Pseudomonas syringae pv. tomato DC3000 Wild type White Rep 4 GSM2715504 Public on Oct 01 2018 Jul 23 2017 Oct 01 2018 RNA 1 Wild type 10 min under White light Pseudomonas syringae pv. tomato str. DC3000 genotype/variation: Wild type culture type: Liquid treatment: White light treatment duration: 10 min Transcriptomic analyses were done with cultures grown in liquid KB medium. Total RNA was extracted from cell cultures collected at O.D.=0.5 after a 10 min treatment with either white, blue or red light. Cultures maintained in the darkness were used as controls. total RNA Total RNA from four biological replicates from each treatment was extracted with TRI Reagent (Ambion) as recommended by the manufacturer. RNA was pre-treated with RNase-free DNase I (Roche) plus RNaseOUT (Invitrogen), followed by purification with RNeasy columns (Qiagen). Cy3 cDNA was labeled with Cy3 by using the SuperScript III indirect cDNA labeling system (Invitrogen) as described in the instruction manual (One-Color Microarray Based Gene Expression Analysis Manual). 223283 600 ng of Cy3 probes were mixed and added to 5 μl of 10x Blocking Agent and Nuclease-free water in a 25 μl reaction. Then, we added 25 μl from 2x GExHybridization buffer and mixed carefully. The samples were placed on ice and quickly loaded onto arrays, hybridized at 65 ºC for 17 h and then washed once in GE wash buffer 1 at room temperature (1 min) and once in GE Wash Buffer 2 at 37 ºC (1 min). Images from Cy3 channel were equilibrated and captured with a High resolution Scanner (Agilent) and spots quantified using Feature Extraction software (Agilent). G3 Gene expression after 10 min white light treatment. For local background correction and normalization, the methods normexp and loess in LIMMA were used, respectively. Log-ratio values were scaled using as scale estimator the median-absolute-value. Linear model methods were used for determining differentially expressed genes. Each probe was tested for changes in expression over replicates by using an empirical Bayes moderated t-statistic. To control the false discovery rate, p-values were corrected by using the method of Benjamani and Hochberg, 1995. The expected false discovery rate (FDR) was controlled to be less than 1%. GPL23823 Emilia,,López Solanilla emilia.lopez@upm.es CBGP-Universidad Politécnica de Madrid (UPM) Parque Científico y Tecnológico UPM, Campus de Montegancedo, Ctra. M-40, km 38 Pozuelo de Alarcon Madrid 28223 Spain ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM2715nnn/GSM2715504/suppl/GSM2715504_White_Rep_4_Raw.txt.gz 15744 Liquid Wild type 10 min White light