Class 7: Linear Models for Micro-arrays

Systems Biology

Andrés Aravena, PhD

October 22, 2021

Something like Gene expression

Today we will study a miRNA micro-array

Each measured value corresponds to the concentration of a miRNA

(in theory, at least)

I may say “gene expression” instead of “miRNA expression”. Please forgive me.

Data looks like this

                AL_T95_blood_w50.ga.cel AL_T96_blood_w50.ga.cel
MIMAT0000121_st                   2.370                   4.250
MIMAT0004519_st                   0.402                   0.482
MIMAT0000122_st                   2.300                   5.870
MIMAT0004520_st                   0.390                   0.633
MIMAT0016979_st                   0.592                   0.486
MIMAT0000123_st                   0.481                   0.568
MIMAT0000124_st                   3.980                   6.960
MIMAT0004521_st                   1.070                   0.595
MIMAT0016980_st                   0.687                   0.608
MIMAT0000125_st                   0.305                   2.970
                AL_T97_blood_w50.ga.cel CCR_T106_blood_w50.ga.cel
MIMAT0000121_st                   4.850                     4.120
MIMAT0004519_st                   0.384                     0.597
MIMAT0000122_st                   6.430                     6.090
MIMAT0004520_st                   0.581                     1.210
MIMAT0016979_st                   0.362                     0.250
MIMAT0000123_st                   0.544                     0.592
MIMAT0000124_st                   7.310                     7.610
MIMAT0004521_st                   0.576                     0.610
MIMAT0016980_st                   0.429                     0.530
MIMAT0000125_st                   2.420                     2.070

Each colum is an array

colnames(norm.data)
 [1] "AL_T95_blood_w50.ga.cel"     "AL_T96_blood_w50.ga.cel"    
 [3] "AL_T97_blood_w50.ga.cel"     "CCR_T106_blood_w50.ga.cel"  
 [5] "CCR_T107_blood_w50.ga.cel"   "CCR_T94_blood_w50.ga.cel"   
 [7] "ICRR_T196_blood_w50.ga.cel"  "ICRR_T197_blood_w50.ga.cel" 
 [9] "ICRR_T79_blood_w50.ga.cel"   "ICRRF_T111_blood_w50.ga.cel"
[11] "ICRRF_T223_blood_w50.ga.cel" "ICRRF_T76_blood_w50.ga.cel" 

We identify each condition

condition <- factor(c(rep("AL",3),rep("CCR",3),rep("ICRR",3),rep("ICRRF",3)))
condition
 [1] AL    AL    AL    CCR   CCR   CCR   ICRR  ICRR  ICRR  ICRRF ICRRF ICRRF
Levels: AL CCR ICRR ICRRF

It is easy to model a single gene

lm(norm.data[1,] ~ condition) |> coef()
   (Intercept)   conditionCCR  conditionICRR conditionICRRF 
       3.82274       -0.73271       -0.03442       -0.76650 

Here (Intercept) is the average expression under AL, and conditionCCR, conditionICRR and conditionICRRF are the differential expression with respect to AL

The design matrix

Last class we discussed how this model is described with a matrix. R can do that matrix for us

model.matrix(norm.data[1,] ~ condition)
                            (Intercept) conditionCCR conditionICRR conditionICRRF
AL_T95_blood_w50.ga.cel               1            0             0              0
AL_T96_blood_w50.ga.cel               1            0             0              0
AL_T97_blood_w50.ga.cel               1            0             0              0
CCR_T106_blood_w50.ga.cel             1            1             0              0
CCR_T107_blood_w50.ga.cel             1            1             0              0
CCR_T94_blood_w50.ga.cel              1            1             0              0
ICRR_T196_blood_w50.ga.cel            1            0             1              0
ICRR_T197_blood_w50.ga.cel            1            0             1              0
ICRR_T79_blood_w50.ga.cel             1            0             1              0
ICRRF_T111_blood_w50.ga.cel           1            0             0              1
ICRRF_T223_blood_w50.ga.cel           1            0             0              1
ICRRF_T76_blood_w50.ga.cel            1            0             0              1
attr(,"assign")
[1] 0 1 1 1
attr(,"contrasts")
attr(,"contrasts")$condition
[1] "contr.treatment"

Without intercept

lm(norm.data[1,] ~ condition+0) |> coef()
   conditionAL   conditionCCR  conditionICRR conditionICRRF 
         3.823          3.090          3.788          3.056 

Here all coefficients are the average expression under each condition

The design matrix

model.matrix( ~ condition+0)
   conditionAL conditionCCR conditionICRR conditionICRRF
1            1            0             0              0
2            1            0             0              0
3            1            0             0              0
4            0            1             0              0
5            0            1             0              0
6            0            1             0              0
7            0            0             1              0
8            0            0             1              0
9            0            0             1              0
10           0            0             0              1
11           0            0             0              1
12           0            0             0              1
attr(,"assign")
[1] 1 1 1 1
attr(,"contrasts")
attr(,"contrasts")$condition
[1] "contr.treatment"

Store the design

design <- model.matrix( ~ condition)
design
   (Intercept) conditionCCR conditionICRR conditionICRRF
1            1            0             0              0
2            1            0             0              0
3            1            0             0              0
4            1            1             0              0
5            1            1             0              0
6            1            1             0              0
7            1            0             1              0
8            1            0             1              0
9            1            0             1              0
10           1            0             0              1
11           1            0             0              1
12           1            0             0              1
attr(,"assign")
[1] 0 1 1 1
attr(,"contrasts")
attr(,"contrasts")$condition
[1] "contr.treatment"

Rename column names

colnames(design) <- levels(condition)
design
   AL CCR ICRR ICRRF
1   1   0    0     0
2   1   0    0     0
3   1   0    0     0
4   1   1    0     0
5   1   1    0     0
6   1   1    0     0
7   1   0    1     0
8   1   0    1     0
9   1   0    1     0
10  1   0    0     1
11  1   0    0     1
12  1   0    0     1
attr(,"assign")
[1] 0 1 1 1
attr(,"contrasts")
attr(,"contrasts")$condition
[1] "contr.treatment"

Fitting the linear model to all genes

library(limma)
fit <- lmFit(norm.data, design)

This is a list of several things, but we do not use it directly

names(fit)
 [1] "coefficients"     "rank"             "assign"           "qr"              
 [5] "df.residual"      "sigma"            "cov.coefficients" "stdev.unscaled"  
 [9] "pivot"            "Amean"            "method"           "design"          

Calculating p-values

Limma provides a statistical model to estimate the variances

It assumes that the noise of all probes have similar variance

The method is called “Empirical Bayes”

eb <- eBayes(fit)

Again a list of several things

names(eb)
 [1] "coefficients"     "rank"             "assign"           "qr"              
 [5] "df.residual"      "sigma"            "cov.coefficients" "stdev.unscaled"  
 [9] "pivot"            "Amean"            "method"           "design"          
[13] "df.prior"         "s2.prior"         "var.prior"        "proportion"      
[17] "s2.post"          "t"                "df.total"         "p.value"         
[21] "lods"             "F"                "F.p.value"       

We do not use these values directly

Top Ten

Instead we use the function

topTable(eb)
                    AL     CCR     ICRR     ICRRF AveExpr      F   P.Value adj.P.Val
MIMAT0000523_st  8.485  0.8469  0.71348 -0.160392   8.835 1096.2 6.392e-13 6.707e-10
MIMAT0020637_st 10.520 -0.5121 -0.42160  0.006145  10.288 1075.0 7.030e-13 6.707e-10
MIMAT0000539_st  7.747  0.1857  1.62009  0.998393   8.449  951.9 1.271e-12 8.086e-10
MIMAT0000522_st  9.320  0.8082  1.33504  0.382160   9.952  883.9 1.824e-12 8.701e-10
MIMAT0003130_st 10.865  0.2240  1.40976  0.228008  11.330  680.9 6.498e-12 2.066e-09
MIMAT0014943_st 10.865  0.2240  1.40976  0.228008  11.330  680.9 6.498e-12 2.066e-09
MIMAT0000221_st  7.747 -0.5157 -0.18510  0.175616   7.615  419.8 6.821e-11 1.859e-08
MIMAT0000521_st  6.502  0.8385  0.07341 -0.498622   6.605  349.8 1.651e-10 3.937e-08
MIMAT0011213_st  7.756 -1.2724 -1.22883 -0.356795   7.042  338.4 1.938e-10 4.109e-08
MIMAT0000383_st  8.792  0.1853 -0.59515 -1.069890   8.422  315.4 2.729e-10 4.752e-08

We can ask for every condition

The second coefficient is CCR v/s AL

topTable(eb, coef=2)
                  logFC AveExpr      t   P.Value adj.P.Val      B
MIMAT0003182_st  3.6380  2.5948  5.462 0.0002994    0.5713 -2.782
MIMAT0024861_st  3.9560  4.9462  4.429 0.0013488    0.9205 -3.065
MIMAT0003499_st  3.8790  7.4007  4.383 0.0014473    0.9205 -3.080
MIMAT0025142_st -0.3749  0.3160 -3.816 0.0035416    0.9869 -3.283
MIMAT0022952_st  0.3116  0.2805  3.626 0.0048162    0.9869 -3.359
MIMAT0014842_st  0.3128  0.7109  3.375 0.0072913    0.9869 -3.466
MIMAT0017051_st -0.2494  0.2142 -3.355 0.0075440    0.9869 -3.475
MIMAT0017255_st  0.2597  0.1254  3.313 0.0080841    0.9869 -3.494
MIMAT0003737_st -0.2006  0.1918 -3.309 0.0081315    0.9869 -3.495
MIMAT0027970_st -2.3755  1.6257 -3.248 0.0090160    0.9869 -3.523

Notice that this time it uses a Students-t test

Seeing more than 10 genes

By default topTable shows 10 genes. We can ask for any number

topTable(eb, coef=2, number = Inf)
                     logFC    AveExpr         t   P.Value adj.P.Val      B
MIMAT0003182_st  3.6380392  2.5947992  5.461870 0.0002994    0.5713 -2.782
MIMAT0024861_st  3.9560290  4.9462427  4.429252 0.0013488    0.9205 -3.065
MIMAT0003499_st  3.8789692  7.4007105  4.383314 0.0014473    0.9205 -3.080
MIMAT0025142_st -0.3749471  0.3159677 -3.815699 0.0035416    0.9869 -3.283
MIMAT0022952_st  0.3115565  0.2804981  3.626482 0.0048162    0.9869 -3.359
MIMAT0014842_st  0.3127530  0.7108909  3.375000 0.0072913    0.9869 -3.466
MIMAT0017051_st -0.2493936  0.2142253 -3.354508 0.0075440    0.9869 -3.475
MIMAT0017255_st  0.2597429  0.1253642  3.312985 0.0080841    0.9869 -3.494
MIMAT0003737_st -0.2006156  0.1918254 -3.309485 0.0081315    0.9869 -3.495
MIMAT0027970_st -2.3754851  1.6256800 -3.247663 0.0090160    0.9869 -3.523
MIMAT0028053_st -0.2885286  0.3294399 -3.128918 0.0110032    0.9869 -3.578
MIMAT0022381_st -0.3112508  0.6726499 -3.043114 0.0127144    0.9869 -3.618
MIMAT0027800_st -0.2235477  0.1376502 -3.029679 0.0130060    0.9869 -3.625
MIMAT0025114_st -0.2792711  0.4100383 -2.941003 0.0151094    0.9869 -3.668
MIMAT0027765_st  0.3192069  0.4497825  2.920476 0.0156438    0.9869 -3.678
MIMAT0009460_st -0.2763771  0.2306513 -2.897225 0.0162723    0.9869 -3.689
MIMAT0004528_st  0.3322297  0.1903511  2.884249 0.0166342    0.9869 -3.696
MIMAT0000130_st -1.7431901  1.4583859 -2.873101 0.0169515    0.9869 -3.701
MIMAT0027724_st -0.7991045  0.5755867 -2.860155 0.0173278    0.9869 -3.707
MIMAT0028441_st -0.2863798  0.3716359 -2.850667 0.0176089    0.9869 -3.712
MIMAT0027983_st -0.3154435  0.2167411 -2.806703 0.0189728    0.9869 -3.734
MIMAT0000248_st -1.6484080  0.7412942 -2.758427 0.0205938    0.9869 -3.758
MIMAT0000653_st  0.1920339  0.3440203  2.712588 0.0222618    0.9869 -3.782
MIMAT0027978_st -0.4028807  0.5479642 -2.689801 0.0231408    0.9869 -3.793
MIMAT0007866_st -0.3450878  0.7666183 -2.654410 0.0245755    0.9869 -3.811
MIMAT0017210_st  0.2772695  0.3511039  2.639958 0.0251866    0.9869 -3.819
MIMAT0014818_st -0.2404322  0.4073962 -2.635306 0.0253866    0.9869 -3.821
MIMAT0024857_st -0.3205886  0.4612988 -2.630102 0.0256121    0.9869 -3.824
MIMAT0014876_st  0.2660588  0.4974177  2.614670 0.0262928    0.9869 -3.832
MIMAT0004573_st  0.1931397  0.2393662  2.595895 0.0271453    0.9869 -3.842
MIMAT0005856_st  2.1161874  2.1162126  2.591627 0.0273429    0.9869 -3.844
MIMAT0004871_st  0.1956567  0.4853727  2.589796 0.0274281    0.9869 -3.845
MIMAT0020642_st -0.2795236  0.3123420 -2.567678 0.0284786    0.9869 -3.856
MIMAT0022370_st  0.2273070  0.3960548  2.560195 0.0288429    0.9869 -3.860
MIMAT0024859_st  1.8652829  1.4578823  2.555556 0.0290712    0.9869 -3.863
MIMAT0002106_st -0.2304201  0.5906431 -2.552937 0.0292008    0.9869 -3.864
MIMAT0017243_st -0.2822373  0.3308539 -2.550363 0.0293288    0.9869 -3.865
MIMAT0017347_st -0.2822373  0.3308539 -2.550363 0.0293288    0.9869 -3.865
MIMAT0014896_st -0.1803800  0.6180693 -2.544896 0.0296024    0.9869 -3.868
MIMAT0028077_st  0.3113522  0.7526295  2.528864 0.0304196    0.9869 -3.877
MIMAT0000672_st -0.1784739  0.2513439 -2.517288 0.0310235    0.9869 -3.883
MIMAT0009456_st -0.7543715  0.6174646 -2.515685 0.0311081    0.9869 -3.883
MIMAT0029889_st -0.2104717  0.0068327 -2.511903 0.0313085    0.9869 -3.885
MIMAT0000156_st -1.1882971  0.6469866 -2.498674 0.0320197    0.9869 -3.892
MIMAT0025078_st -0.2580285  0.5524441 -2.494593 0.0322423    0.9869 -3.895
MIMAT0004782_st  0.2445541  0.3731671  2.479530 0.0330772    0.9869 -3.902
MIMAT0027883_st -0.2706816  0.5838178 -2.471026 0.0335579    0.9869 -3.907
MIMAT0027775_st -0.2448609  0.4418762 -2.465265 0.0338876    0.9869 -3.910
MIMAT0000513_st -2.0648727  1.1977118 -2.460885 0.0341403    0.9869 -3.912
MIMAT0004662_st  0.3060468  0.7966416  2.454955 0.0344854    0.9869 -3.915
MIMAT0014959_st -0.2986662  0.2307641 -2.441404 0.0352871    0.9869 -3.923
MIMAT0028086_st -0.1819961  0.5800176 -2.433570 0.0357589    0.9869 -3.927
MIMAT0004665_st -0.3038802  0.1237273 -2.422370 0.0364443    0.9869 -3.933
MIMAT0003187_st -1.6222111  0.9418695 -2.416626 0.0368008    0.9869 -3.936
MIMAT0027732_st  0.3008940  0.3182401  2.416225 0.0368257    0.9869 -3.936
MIMAT0004525_st -0.2424616  0.2765412 -2.383593 0.0389184    0.9869 -3.953
MIMAT0003181_st -0.2094032  0.1935017 -2.347504 0.0413688    0.9869 -3.973
MIMAT0011213_st -1.2724456  7.0418497 -2.343433 0.0416545    0.9869 -3.975
MIMAT0001632_st -2.2915505  6.4685964 -2.338848 0.0419786    0.9869 -3.977
MIMAT0017022_st -0.1937495  0.3394556 -2.302640 0.0446261    0.9869 -3.997
MIMAT0016985_st -0.1520502  0.2575218 -2.280862 0.0462962    0.9869 -4.008
MIMAT0027880_st  0.3147787  0.8273944  2.269950 0.0471557    0.9869 -4.014
MIMAT0004879_st  0.3163073  0.2656124  2.261881 0.0478013    0.9869 -4.018
MIMAT0004187_st  1.5055865  6.1379552  2.242796 0.0493625    0.9869 -4.029
MIMAT0000523_st  0.8469332  8.8347965  2.241582 0.0494635    0.9869 -4.029
MIMAT0029818_st -0.1551275  0.1994756 -2.239419 0.0496439    0.9869 -4.031
MIMAT0017343_st -0.2379481  0.4290004 -2.230366 0.0504058    0.9869 -4.035
MIMAT0000597_st  0.2940827  0.5157739  2.229080 0.0505149    0.9869 -4.036
MIMAT0003897_st  0.1957889  0.4258319  2.226180 0.0507620    0.9869 -4.038
MIMAT0000528_st -2.0996276  2.1580278 -2.192856 0.0536852    0.9869 -4.056
MIMAT0017009_st  0.1887872  0.4092422  2.189763 0.0539646    0.9869 -4.057
MIMAT0028088_st -0.2122008  0.5747037 -2.184725 0.0544227    0.9869 -4.060
MIMAT0027725_st -0.3675277  0.3145628 -2.180119 0.0548449    0.9869 -4.063
MIMAT0028432_st -0.2531502  0.5427220 -2.177892 0.0550502    0.9869 -4.064
MIMAT0003462_st -0.2000636  0.1682847 -2.172972 0.0555062    0.9869 -4.066
MIMAT0025106_st -0.3228883  0.4787261 -2.168497 0.0559241    0.9869 -4.069
MIMAT0019136_st -0.2061593  0.6143565 -2.167115 0.0560538    0.9869 -4.070
MIMAT0027966_st -0.2476122  0.6450389 -2.148644 0.0578151    0.9869 -4.080
MIMAT0017021_st -0.1817726  0.2637920 -2.141621 0.0584987    0.9869 -4.083
MIMAT0016983_st  0.1834425  0.1685592  2.139764 0.0586807    0.9869 -4.084
MIMAT0000124_st  1.7424315  6.8283304  2.128649 0.0597817    0.9869 -4.090
MIMAT0004547_st -0.1326423  0.3193194 -2.127612 0.0598855    0.9869 -4.091
MIMAT0004684_st -0.1405597  0.2695488 -2.125075 0.0601399    0.9869 -4.092
MIMAT0028446_st -0.1671729  0.2620657 -2.123594 0.0602890    0.9869 -4.093
MIMAT0001081_st  0.2126237  0.3252995  2.108251 0.0618539    0.9869 -4.101
MIMAT0017275_st -0.2239218  0.2906711 -2.108156 0.0618637    0.9869 -4.102
MIMAT0000128_st -2.0081930  2.6647325 -2.105576 0.0621307    0.9869 -4.103
MIMAT0020624_st -0.2208141  0.3218937 -2.098504 0.0628682    0.9869 -4.107
MIMAT0014807_st -0.1855725  0.2337073 -2.092686 0.0634812    0.9869 -4.110
MIMAT0000595_st -0.7382192  0.7609687 -2.087817 0.0639987    0.9869 -4.113
MIMAT0000375_st  0.4757793  0.9052619  2.081750 0.0646490    0.9869 -4.116
MIMAT0000648_st -0.2832839  0.2955861 -2.077239 0.0651366    0.9869 -4.118
MIMAT0004858_st -0.2000663  0.1265310 -2.071543 0.0657574    0.9869 -4.121
MIMAT0004846_st -0.2349275  0.2394177 -2.070999 0.0658169    0.9869 -4.122
MIMAT0004526_st  0.1470720  0.4175828  2.068564 0.0660842    0.9869 -4.123
MIMAT0003168_st -0.1863357  0.1265237 -2.063909 0.0665981    0.9869 -4.125
MIMAT0027843_st  0.2217770  0.5449116  2.059422 0.0670970    0.9869 -4.128
MIMAT0000769_st -0.2573032  0.3890684 -2.053242 0.0677901    0.9869 -4.131
MIMAT0017261_st  0.2856855  0.4429363  2.030482 0.0704016    0.9869 -4.144
MIMAT0017257_st -0.1834212  0.4049434 -2.024221 0.0711366    0.9869 -4.147
MIMAT0014837_st -0.2056411  0.1505220 -2.022804 0.0713039    0.9869 -4.148
MIMAT0019355_st -0.4014599  0.5558786 -2.017386 0.0719472    0.9869 -4.151
MIMAT0017248_st -0.1636877  0.3123408 -2.015293 0.0721972    0.9869 -4.152
MIMAT0000667_st  0.2006634  0.3660203  2.014710 0.0722670    0.9869 -4.152
MIMAT0028020_st -1.0149931  1.0158601 -2.007127 0.0731803    0.9869 -4.156
MIMAT0022372_st -0.6180256  1.2504536 -2.003824 0.0735816    0.9869 -4.158
MIMAT0017039_st  1.7366387  6.6746443  2.003210 0.0736564    0.9869 -4.158
MIMAT0000658_st -1.8877287  2.3867925 -2.002507 0.0737423    0.9869 -4.159
MIMAT0014861_st  0.1910488  0.3603710  2.001340 0.0738848    0.9869 -4.159
MIMAT0027915_st -0.1778824  0.0891270 -1.998110 0.0742807    0.9869 -4.161
MIMAT0029799_st  1.6137958  5.9053151  1.991342 0.0751169    0.9869 -4.165
MIMAT0004633_st  0.1819901  0.7541098  1.984828 0.0759302    0.9869 -4.168
MIMAT0027816_st  0.2582207  0.5125412  1.984459 0.0759764    0.9869 -4.169
MIMAT0029858_st -0.4087652  0.5704812 -1.983840 0.0760542    0.9869 -4.169
MIMAT0028039_st  0.3287858  0.4591102  1.983158 0.0761399    0.9869 -4.169
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MIMAT0004843_st -0.0021965  0.4447281 -0.017959 0.9860331    0.9989 -4.874
MIMAT0017349_st  0.0019572  0.1831789  0.017328 0.9865239    0.9989 -4.874
MIMAT0019340_st  0.0032923  0.7607252  0.017195 0.9866269    0.9989 -4.874
MIMAT0009412_st -0.0029801  1.0720632 -0.016746 0.9869760    0.9989 -4.874
MIMAT0004899_st  0.0144883  2.1546107  0.016196 0.9874039    0.9989 -4.874
MIMAT0004891_st -0.0020380  0.1229170 -0.015686 0.9878003    0.9989 -4.874
MIMAT0022505_st  0.0016217  0.3957141  0.015404 0.9880194    0.9989 -4.874
MIMAT0003782_st -0.0120910  2.3936441 -0.014401 0.9887994    0.9989 -4.874
MIMAT0004647_st  0.0019143  0.2059457  0.014340 0.9888471    0.9989 -4.874
MIMAT0028450_st  0.0020328  0.5051371  0.014009 0.9891046    0.9989 -4.874
MIMAT0005845_st -0.0015176  0.3362791 -0.013556 0.9894573    0.9989 -4.874
MIMAT0016998_st  0.0078752  1.2455450  0.012400 0.9903556    0.9989 -4.874
MIMAT0022729_st -0.0016421  0.3457524 -0.011521 0.9910394    0.9989 -4.874
MIMAT0000546_st  0.0075365  7.3185030  0.011460 0.9910872    0.9989 -4.874
MIMAT0028002_st  0.0016086  0.4961236  0.011335 0.9911840    0.9989 -4.874
MIMAT0025174_st  0.0013803  0.0205888  0.010696 0.9916811    0.9989 -4.874
MIMAT0028042_st -0.0018642  0.3991708 -0.010259 0.9920211    0.9989 -4.874
MIMAT0025137_st -0.0011613  0.2547694 -0.009815 0.9923660    0.9989 -4.874
MIMAT0014868_st -0.0019000  0.6517041 -0.008479 0.9934055    0.9989 -4.874
MIMAT0000164_st  0.0004118  0.2168983  0.005712 0.9955577    0.9989 -4.874
MIMAT0004631_st  0.0007168  0.6023453  0.005613 0.9956348    0.9989 -4.874
MIMAT0003889_st -0.0003897  0.0469878 -0.005279 0.9958942    0.9989 -4.874
MIMAT0025158_st  0.0004747  0.3078754  0.004362 0.9966077    0.9989 -4.874
MIMAT0000674_st -0.0005339  0.5830866 -0.004316 0.9966428    0.9989 -4.874
MIMAT0025150_st -0.0003082  0.0340002 -0.004295 0.9966596    0.9989 -4.874
MIMAT0012774_st -0.0004963  0.3026882 -0.003994 0.9968933    0.9989 -4.874
MIMAT0029852_st  0.0001885  0.2543605  0.003164 0.9975394    0.9989 -4.874
MIMAT0015642_st -0.0004779  0.2494059 -0.003151 0.9975490    0.9989 -4.874
MIMAT0004868_st -0.0002231  0.1884718 -0.002635 0.9979504    0.9989 -4.874
MIMAT0027890_st  0.0005846  0.4822407  0.002060 0.9983979    0.9989 -4.874
MIMAT0028010_st  0.0011467  2.9789566  0.001355 0.9989457    0.9989 -4.874

We can also make some plots

plotMD(eb, coef = 2)

p-value v/s log-fold-change

plot(P.Value ~ logFC, topTable(eb, coef=2, number = Inf), pch=16, cex=0.3)

Better use log10

plot(log10(P.Value) ~ logFC, topTable(eb, coef=2, number = Inf), pch=16, cex=0.3)

Let’s change sign

plot(-log10(P.Value) ~ logFC, topTable(eb, coef=2, number = Inf), pch=16, cex=0.3)

This is called volcano plot

Volcano plot

volcanoplot(eb, coef = 2)

Volcano plot

volcanoplot(eb, coef = 2)
abline(h=-log10(0.05))

GEO2R makes the same plots

Take a look at https://www.ncbi.nlm.nih.gov/geo/info/geo2r.html

These plots are explained there

And you will see other plots that may be useful

More conditions

Now for the real fun

Loading required package: Matrix
full.data[1:5,]
                AL_T3_Brain_w80.ga.cel AL_T67_Brain_w80.ga.cel
MIMAT0000121_st                 7.2959                  7.9893
MIMAT0004519_st                 1.7060                  1.1669
MIMAT0000122_st                 8.3674                  8.5103
MIMAT0004520_st                 1.7753                  0.5343
MIMAT0016979_st                 0.5324                  0.7117
                AL_T123_Brain_w80.ga.cel CCR_T33_Brain_w80.ga.cel
MIMAT0000121_st                   7.3608                   7.8563
MIMAT0004519_st                   1.0579                   1.4179
MIMAT0000122_st                   8.1222                   8.7744
MIMAT0004520_st                   0.7154                   0.9583
MIMAT0016979_st                   0.7006                   0.6641
                CCR_T38_Brain_w80.ga.cel CCR_T125_Brain_w80.ga.cel
MIMAT0000121_st                   6.7607                    6.6513
MIMAT0004519_st                   1.6417                    1.2777
MIMAT0000122_st                   7.5414                    7.8640
MIMAT0004520_st                   1.1278                    0.8770
MIMAT0016979_st                   0.3608                    0.3895
                ICRR_T137_Brain_w80.ga.cel ICRR_T70_Brain_w80.ga.cel
MIMAT0000121_st                     7.4784                    5.5468
MIMAT0004519_st                     1.0750                    1.3626
MIMAT0000122_st                     8.3045                    6.2543
MIMAT0004520_st                     1.1987                    1.4316
MIMAT0016979_st                     0.5544                    0.7617
                ICRR_T22_Brain_w80.ga.cel ICRRF_T117_Brain_w80.ga.cel
MIMAT0000121_st                    7.4300                      6.6186
MIMAT0004519_st                    1.2333                      1.1818
MIMAT0000122_st                    7.9575                      7.2884
MIMAT0004520_st                    0.5289                      0.9234
MIMAT0016979_st                    0.6602                      0.5859
                ICRRF_T116_Brain_w80.ga.cel ICRRF_T25_Brain_w80.ga.cel
MIMAT0000121_st                      7.2304                     7.0761
MIMAT0004519_st                      1.5379                     1.9485
MIMAT0000122_st                      8.4287                     8.0246
MIMAT0004520_st                      1.3546                     1.6478
MIMAT0016979_st                      0.4001                     0.6143
                AL_T95_blood_w50.ga.cel AL_T96_blood_w50.ga.cel
MIMAT0000121_st                  2.5598                  7.6623
MIMAT0004519_st                  1.0340                  1.1708
MIMAT0000122_st                  5.1940                  9.6824
MIMAT0004520_st                  0.7467                  1.2821
MIMAT0016979_st                  1.2478                  0.8626
                AL_T97_blood_w50.ga.cel CCR_T94_blood_w50.ga.cel
MIMAT0000121_st                  8.2261                   3.4159
MIMAT0004519_st                  1.2509                   0.9768
MIMAT0000122_st                 10.3019                   6.5261
MIMAT0004520_st                  1.0199                   0.6930
MIMAT0016979_st                  0.5789                   1.5115
                CCR_T106_blood_w50.ga.cel CCR_T107_blood_w50.ga.cel
MIMAT0000121_st                    7.5862                    6.3770
MIMAT0004519_st                    1.1677                    0.7518
MIMAT0000122_st                    9.9065                    9.7258
MIMAT0004520_st                    2.5481                    1.1791
MIMAT0016979_st                    0.9358                    0.5442
                ICRR_T79_blood_w50.ga.cel ICRR_T196_blood_w50.ga.cel
MIMAT0000121_st                     7.519                     6.3644
MIMAT0004519_st                     1.157                     0.9727
MIMAT0000122_st                     8.836                     8.4647
MIMAT0004520_st                     1.903                     1.0104
MIMAT0016979_st                     1.017                     0.9368
                ICRR_T197_blood_w50.ga.cel ICRRF_T76_blood_w50.ga.cel
MIMAT0000121_st                     6.9660                     4.2863
MIMAT0004519_st                     1.3095                     0.7683
MIMAT0000122_st                     8.7879                     3.9935
MIMAT0004520_st                     0.7914                     0.9234
MIMAT0016979_st                     0.8620                     1.1623
                ICRRF_T111_blood_w50.ga.cel ICRRF_T223_blood_w50.ga.cel
MIMAT0000121_st                      7.0538                      6.4261
MIMAT0004519_st                      1.4682                      0.9592
MIMAT0000122_st                      8.4773                      8.9032
MIMAT0004520_st                      0.9048                      0.8973
MIMAT0016979_st                      0.7906                      0.7117
                AL_T66_Blood_w80.ga.cel AL_T67_Blood_w80.ga.cel
MIMAT0000121_st                  7.2005                  8.0572
MIMAT0004519_st                  0.7476                  0.9857
MIMAT0000122_st                  9.7348                 10.1907
MIMAT0004520_st                  0.6506                  0.8755
MIMAT0016979_st                  0.7161                  0.6364
                AL_T123_Blood_w80.ga.cel CCR_T38_Blood_w80.ga.cel
MIMAT0000121_st                   7.5301                   7.5029
MIMAT0004519_st                   0.9193                   1.2757
MIMAT0000122_st                   9.9642                   9.9447
MIMAT0004520_st                   1.6289                   1.8655
MIMAT0016979_st                   0.5996                   0.6964
                CCR_T125_Blood_w80.ga.cel CCR_T126_Blood_w80.ga.cel
MIMAT0000121_st                    8.0007                    7.6984
MIMAT0004519_st                    0.9219                    0.9412
MIMAT0000122_st                    9.8461                    9.1968
MIMAT0004520_st                    0.9033                    0.9782
MIMAT0016979_st                    0.7117                    0.9738
                ICRR_T70_Blood_w80.ga.cel ICRR_T137_Blood_w80.ga.cel
MIMAT0000121_st                    7.6554                     6.2819
MIMAT0004519_st                    1.0955                     0.8162
MIMAT0000122_st                   10.0833                     7.3940
MIMAT0004520_st                    0.9416                     0.8284
MIMAT0016979_st                    0.8053                     0.7117
                ICRR_T138_Blood_w80.ga.cel ICRRF_T116_Blood_w80.ga.cel
MIMAT0000121_st                     4.8502                      4.8367
MIMAT0004519_st                     0.7810                      0.9209
MIMAT0000122_st                     7.4459                      8.0622
MIMAT0004520_st                     0.6301                      0.8555
MIMAT0016979_st                     0.7511                      0.8894
                ICRRF_T117_Blood_w80.ga.cel ICRRF_T129_Blood_w80.ga.cel
MIMAT0000121_st                      7.6579                      6.7005
MIMAT0004519_st                      0.9544                      0.8747
MIMAT0000122_st                     10.1353                      8.8342
MIMAT0004520_st                      0.6773                      1.5271
MIMAT0016979_st                      0.5538                      1.0597
                AL_T95_MFP_w50.ga.cel AL_T96_MFP_w50.ga.cel AL_T97_MFP_w50.ga.cel
MIMAT0000121_st                6.2962                7.5608                4.6226
MIMAT0004519_st                2.4143                1.5610                1.0915
MIMAT0000122_st                8.5038               10.3497                6.5139
MIMAT0004520_st                1.3980                1.2467                0.4558
MIMAT0016979_st                0.8277                0.8672                0.7933
                CCR_T94_MFP_w50.ga.cel CCR_T106_MFP_w50.ga.cel
MIMAT0000121_st                 7.8290                  8.4951
MIMAT0004519_st                 0.8879                  2.3016
MIMAT0000122_st                 8.7140                  9.9587
MIMAT0004520_st                 0.9090                  0.9978
MIMAT0016979_st                 0.8643                  0.5924
                CCR_T107_MFP_w50.ga.cel ICRR_T130_MFP_w50.ga.cel
MIMAT0000121_st                  6.1267                   9.2525
MIMAT0004519_st                  1.2592                   1.6226
MIMAT0000122_st                  8.6674                  10.2766
MIMAT0004520_st                  1.1526                   1.1623
MIMAT0016979_st                  0.6349                   0.5819
                ICRR_T133_MFP_w50.ga.cel ICRR_T134_MFP_w50.ga.cel
MIMAT0000121_st                   8.3597                   6.7241
MIMAT0004519_st                   0.7009                   0.9235
MIMAT0000122_st                   9.3833                   9.1655
MIMAT0004520_st                   1.0327                   0.6745
MIMAT0016979_st                   0.7586                   1.1597
                ICRRF_T76_MFP_w50.ga.cel ICRRF_T77_MFP_w50.ga.cel
MIMAT0000121_st                   7.5906                   7.6464
MIMAT0004519_st                   2.1235                   1.8892
MIMAT0000122_st                   9.0412                   9.2060
MIMAT0004520_st                   1.1536                   0.5971
MIMAT0016979_st                   0.5372                   0.7746
                ICRRF_T110_MFP_w50.ga.cel AL_T1_MFP_w80.ga.cel AL_T3_MFP_w80.ga.cel
MIMAT0000121_st                    1.3093               6.7281               6.4008
MIMAT0004519_st                    1.1818               2.9889               1.1818
MIMAT0000122_st                    5.3274               8.0821               8.7522
MIMAT0004520_st                    0.9113               0.9754               0.7597
MIMAT0016979_st                    0.7131               0.7745               0.5694
                AL_T123_MFP_w80.ga.cel CCR_T33_MFP_w80.ga.cel CCR_T34_MFP_w80.ga.cel
MIMAT0000121_st                 3.9365                 5.7574                 7.4529
MIMAT0004519_st                 0.8603                 1.0286                 1.3239
MIMAT0000122_st                 5.0478                 7.4141                 8.6882
MIMAT0004520_st                 0.6070                 1.0974                 0.8858
MIMAT0016979_st                 1.2471                 0.7277                 0.7117
                CCR_T37_MFP_w80.ga.cel ICRR_T20_MFP_w80.ga.cel
MIMAT0000121_st                 6.6529                  6.9590
MIMAT0004519_st                 2.4251                  1.6097
MIMAT0000122_st                 8.8301                  8.7409
MIMAT0004520_st                 0.9306                  0.9600
MIMAT0016979_st                 0.4052                  0.6025
                ICRR_T44_MFP_w80.ga.cel ICRR_T71_MFP_w80.ga.cel
MIMAT0000121_st                   5.379                  4.9163
MIMAT0004519_st                   1.843                  1.1635
MIMAT0000122_st                   9.111                  9.1740
MIMAT0004520_st                   1.027                  0.7430
MIMAT0016979_st                   1.233                  0.3506
                ICRRF_T25_MFP_w80.ga.cel ICRRF_T116_MFP_w80.ga.cel
MIMAT0000121_st                   3.0247                    7.1163
MIMAT0004519_st                   0.9109                    1.1169
MIMAT0000122_st                   5.6410                    9.3181
MIMAT0004520_st                   0.6286                    0.7398
MIMAT0016979_st                   0.6094                    0.9966
                ICRRF_T117_MFP_w80.ga.cel BASELINE_T233_Brain.ga.cel
MIMAT0000121_st                    1.6217                      6.534
MIMAT0004519_st                    1.1589                      1.661
MIMAT0000122_st                    2.3852                      7.868
MIMAT0004520_st                    0.8569                      1.547
MIMAT0016979_st                    0.9405                      0.677
                BASELINE_T234_Brain.ga.cel BASELINE_T242_Brain.ga.cel
MIMAT0000121_st                     7.2845                     7.9374
MIMAT0004519_st                     0.9523                     1.8997
MIMAT0000122_st                     8.2988                     8.7130
MIMAT0004520_st                     0.8420                     1.1825
MIMAT0016979_st                     0.4591                     0.6579
                BASELINE_T234_blood.ga.cel BASELINE_T242_blood.ga.cel
MIMAT0000121_st                      3.935                     2.2750
MIMAT0004519_st                      1.055                     0.9957
MIMAT0000122_st                      8.435                     5.3831
MIMAT0004520_st                      0.890                     0.7429
MIMAT0016979_st                      1.482                     1.4299
                BASELINE_T251_blood.ga.cel BASELINE_T190_MFP.ga.cel
MIMAT0000121_st                     7.5224                   6.8341
MIMAT0004519_st                     1.1701                   1.6217
MIMAT0000122_st                     9.5162                   9.5662
MIMAT0004520_st                     0.7948                   0.9234
MIMAT0016979_st                     0.8660                   0.5370
                BASELINE_T193_MFP.ga.cel BASELINE_T242_MFP.ga.cel
MIMAT0000121_st                   8.6298                   3.3204
MIMAT0004519_st                   1.2583                   0.8027
MIMAT0000122_st                  10.1434                   6.4800
MIMAT0004520_st                   0.7874                   0.8490
MIMAT0016979_st                   0.6221                   0.5855
                AL_T95_Brain_w50.CEL AL_T96_Brain_w50.CEL AL_T207_Brain_w50.CEL
MIMAT0000121_st               8.9473                9.090                8.9971
MIMAT0004519_st               1.5473                1.102                1.2903
MIMAT0000122_st               9.2364                9.431                9.5197
MIMAT0004520_st               1.0595                1.226                0.7008
MIMAT0016979_st               0.4354                1.192                0.5528
                CCR_T106_Brain_w50.CEL CCR_T184_brain_w50.CEL CCR_T185_Brain_w50.CEL
MIMAT0000121_st                 8.6099                 9.1495                 9.0157
MIMAT0004519_st                 1.7648                 1.1140                 2.1938
MIMAT0000122_st                 9.1907                 9.3068                 9.3631
MIMAT0004520_st                 0.7404                 0.8062                 1.2632
MIMAT0016979_st                 0.3063                 0.3044                 0.2539
                ICRR_T130_brain_W50.CEL ICRR_T134_Brain_w50.CEL
MIMAT0000121_st                  8.8949                  8.5332
MIMAT0004519_st                  1.5204                  1.6677
MIMAT0000122_st                  9.2183                  9.1968
MIMAT0004520_st                  1.4007                  0.9033
MIMAT0016979_st                  0.6797                  0.9487
                ICRR_T196_brain_W50.CEL ICRRF_T200_brain_W50.CEL
MIMAT0000121_st                  8.7697                   8.7830
MIMAT0004519_st                  1.9516                   1.6719
MIMAT0000122_st                  9.2435                   8.9989
MIMAT0004520_st                  2.5925                   0.7915
MIMAT0016979_st                  0.9883                   0.6796
                ICRRF_T223_brain_W50.CEL ICRRF_T225_brain_W50.CEL
MIMAT0000121_st                   8.5453                   9.1367
MIMAT0004519_st                   1.3829                   1.8936
MIMAT0000122_st                   9.1565                   9.3867
MIMAT0004520_st                   0.7569                   1.5420
MIMAT0016979_st                   0.5245                   0.6182

Much more columns

colnames(full.data)
 [1] "AL_T3_Brain_w80.ga.cel"      "AL_T67_Brain_w80.ga.cel"    
 [3] "AL_T123_Brain_w80.ga.cel"    "CCR_T33_Brain_w80.ga.cel"   
 [5] "CCR_T38_Brain_w80.ga.cel"    "CCR_T125_Brain_w80.ga.cel"  
 [7] "ICRR_T137_Brain_w80.ga.cel"  "ICRR_T70_Brain_w80.ga.cel"  
 [9] "ICRR_T22_Brain_w80.ga.cel"   "ICRRF_T117_Brain_w80.ga.cel"
[11] "ICRRF_T116_Brain_w80.ga.cel" "ICRRF_T25_Brain_w80.ga.cel" 
[13] "AL_T95_blood_w50.ga.cel"     "AL_T96_blood_w50.ga.cel"    
[15] "AL_T97_blood_w50.ga.cel"     "CCR_T94_blood_w50.ga.cel"   
[17] "CCR_T106_blood_w50.ga.cel"   "CCR_T107_blood_w50.ga.cel"  
[19] "ICRR_T79_blood_w50.ga.cel"   "ICRR_T196_blood_w50.ga.cel" 
[21] "ICRR_T197_blood_w50.ga.cel"  "ICRRF_T76_blood_w50.ga.cel" 
[23] "ICRRF_T111_blood_w50.ga.cel" "ICRRF_T223_blood_w50.ga.cel"
[25] "AL_T66_Blood_w80.ga.cel"     "AL_T67_Blood_w80.ga.cel"    
[27] "AL_T123_Blood_w80.ga.cel"    "CCR_T38_Blood_w80.ga.cel"   
[29] "CCR_T125_Blood_w80.ga.cel"   "CCR_T126_Blood_w80.ga.cel"  
[31] "ICRR_T70_Blood_w80.ga.cel"   "ICRR_T137_Blood_w80.ga.cel" 
[33] "ICRR_T138_Blood_w80.ga.cel"  "ICRRF_T116_Blood_w80.ga.cel"
[35] "ICRRF_T117_Blood_w80.ga.cel" "ICRRF_T129_Blood_w80.ga.cel"
[37] "AL_T95_MFP_w50.ga.cel"       "AL_T96_MFP_w50.ga.cel"      
[39] "AL_T97_MFP_w50.ga.cel"       "CCR_T94_MFP_w50.ga.cel"     
[41] "CCR_T106_MFP_w50.ga.cel"     "CCR_T107_MFP_w50.ga.cel"    
[43] "ICRR_T130_MFP_w50.ga.cel"    "ICRR_T133_MFP_w50.ga.cel"   
[45] "ICRR_T134_MFP_w50.ga.cel"    "ICRRF_T76_MFP_w50.ga.cel"   
[47] "ICRRF_T77_MFP_w50.ga.cel"    "ICRRF_T110_MFP_w50.ga.cel"  
[49] "AL_T1_MFP_w80.ga.cel"        "AL_T3_MFP_w80.ga.cel"       
[51] "AL_T123_MFP_w80.ga.cel"      "CCR_T33_MFP_w80.ga.cel"     
[53] "CCR_T34_MFP_w80.ga.cel"      "CCR_T37_MFP_w80.ga.cel"     
[55] "ICRR_T20_MFP_w80.ga.cel"     "ICRR_T44_MFP_w80.ga.cel"    
[57] "ICRR_T71_MFP_w80.ga.cel"     "ICRRF_T25_MFP_w80.ga.cel"   
[59] "ICRRF_T116_MFP_w80.ga.cel"   "ICRRF_T117_MFP_w80.ga.cel"  
[61] "BASELINE_T233_Brain.ga.cel"  "BASELINE_T234_Brain.ga.cel" 
[63] "BASELINE_T242_Brain.ga.cel"  "BASELINE_T234_blood.ga.cel" 
[65] "BASELINE_T242_blood.ga.cel"  "BASELINE_T251_blood.ga.cel" 
[67] "BASELINE_T190_MFP.ga.cel"    "BASELINE_T193_MFP.ga.cel"   
[69] "BASELINE_T242_MFP.ga.cel"    "AL_T95_Brain_w50.CEL"       
[71] "AL_T96_Brain_w50.CEL"        "AL_T207_Brain_w50.CEL"      
[73] "CCR_T106_Brain_w50.CEL"      "CCR_T184_brain_w50.CEL"     
[75] "CCR_T185_Brain_w50.CEL"      "ICRR_T130_brain_W50.CEL"    
[77] "ICRR_T134_Brain_w50.CEL"     "ICRR_T196_brain_W50.CEL"    
[79] "ICRRF_T200_brain_W50.CEL"    "ICRRF_T223_brain_W50.CEL"   
[81] "ICRRF_T225_brain_W50.CEL"   

All possible conditions

colnames(full.data) |> 
    str_remove('.CEL') |> 
    str_remove('.ga.cel') |> 
    str_split_fixed("_",4) -> features
features <- as.data.frame(features)
colnames(features) <- c("diet","replica","tissue","age")
features
       diet replica tissue age
1        AL      T3  Brain w80
2        AL     T67  Brain w80
3        AL    T123  Brain w80
4       CCR     T33  Brain w80
5       CCR     T38  Brain w80
6       CCR    T125  Brain w80
7      ICRR    T137  Brain w80
8      ICRR     T70  Brain w80
9      ICRR     T22  Brain w80
10    ICRRF    T117  Brain w80
11    ICRRF    T116  Brain w80
12    ICRRF     T25  Brain w80
13       AL     T95  blood w50
14       AL     T96  blood w50
15       AL     T97  blood w50
16      CCR     T94  blood w50
17      CCR    T106  blood w50
18      CCR    T107  blood w50
19     ICRR     T79  blood w50
20     ICRR    T196  blood w50
21     ICRR    T197  blood w50
22    ICRRF     T76  blood w50
23    ICRRF    T111  blood w50
24    ICRRF    T223  blood w50
25       AL     T66  Blood w80
26       AL     T67  Blood w80
27       AL    T123  Blood w80
28      CCR     T38  Blood w80
29      CCR    T125  Blood w80
30      CCR    T126  Blood w80
31     ICRR     T70  Blood w80
32     ICRR    T137  Blood w80
33     ICRR    T138  Blood w80
34    ICRRF    T116  Blood w80
35    ICRRF    T117  Blood w80
36    ICRRF    T129  Blood w80
37       AL     T95    MFP w50
38       AL     T96    MFP w50
39       AL     T97    MFP w50
40      CCR     T94    MFP w50
41      CCR    T106    MFP w50
42      CCR    T107    MFP w50
43     ICRR    T130    MFP w50
44     ICRR    T133    MFP w50
45     ICRR    T134    MFP w50
46    ICRRF     T76    MFP w50
47    ICRRF     T77    MFP w50
48    ICRRF    T110    MFP w50
49       AL      T1    MFP w80
50       AL      T3    MFP w80
51       AL    T123    MFP w80
52      CCR     T33    MFP w80
53      CCR     T34    MFP w80
54      CCR     T37    MFP w80
55     ICRR     T20    MFP w80
56     ICRR     T44    MFP w80
57     ICRR     T71    MFP w80
58    ICRRF     T25    MFP w80
59    ICRRF    T116    MFP w80
60    ICRRF    T117    MFP w80
61 BASELINE    T233  Brain    
62 BASELINE    T234  Brain    
63 BASELINE    T242  Brain    
64 BASELINE    T234  blood    
65 BASELINE    T242  blood    
66 BASELINE    T251  blood    
67 BASELINE    T190    MFP    
68 BASELINE    T193    MFP    
69 BASELINE    T242    MFP    
70       AL     T95  Brain w50
71       AL     T96  Brain w50
72       AL    T207  Brain w50
73      CCR    T106  Brain w50
74      CCR    T184  brain w50
75      CCR    T185  Brain w50
76     ICRR    T130  brain W50
77     ICRR    T134  Brain w50
78     ICRR    T196  brain W50
79    ICRRF    T200  brain W50
80    ICRRF    T223  brain W50
81    ICRRF    T225  brain W50

Make a design matrix

features$tissue <- tolower(features$tissue)
design2 <- model.matrix(~ 0 + diet:age:tissue, data=features)
design2
   dietAL:age:tissueblood dietBASELINE:age:tissueblood dietCCR:age:tissueblood
1                       0                            0                       0
2                       0                            0                       0
3                       0                            0                       0
4                       0                            0                       0
5                       0                            0                       0
6                       0                            0                       0
7                       0                            0                       0
8                       0                            0                       0
9                       0                            0                       0
10                      0                            0                       0
11                      0                            0                       0
12                      0                            0                       0
13                      0                            0                       0
14                      0                            0                       0
15                      0                            0                       0
16                      0                            0                       0
17                      0                            0                       0
18                      0                            0                       0
19                      0                            0                       0
20                      0                            0                       0
21                      0                            0                       0
22                      0                            0                       0
23                      0                            0                       0
24                      0                            0                       0
25                      0                            0                       0
26                      0                            0                       0
27                      0                            0                       0
28                      0                            0                       0
29                      0                            0                       0
30                      0                            0                       0
31                      0                            0                       0
32                      0                            0                       0
33                      0                            0                       0
34                      0                            0                       0
35                      0                            0                       0
36                      0                            0                       0
37                      0                            0                       0
38                      0                            0                       0
39                      0                            0                       0
40                      0                            0                       0
41                      0                            0                       0
42                      0                            0                       0
43                      0                            0                       0
44                      0                            0                       0
45                      0                            0                       0
46                      0                            0                       0
47                      0                            0                       0
48                      0                            0                       0
49                      0                            0                       0
50                      0                            0                       0
51                      0                            0                       0
52                      0                            0                       0
53                      0                            0                       0
54                      0                            0                       0
55                      0                            0                       0
56                      0                            0                       0
57                      0                            0                       0
58                      0                            0                       0
59                      0                            0                       0
60                      0                            0                       0
61                      0                            0                       0
62                      0                            0                       0
63                      0                            0                       0
64                      0                            1                       0
65                      0                            1                       0
66                      0                            1                       0
67                      0                            0                       0
68                      0                            0                       0
69                      0                            0                       0
70                      0                            0                       0
71                      0                            0                       0
72                      0                            0                       0
73                      0                            0                       0
74                      0                            0                       0
75                      0                            0                       0
76                      0                            0                       0
77                      0                            0                       0
78                      0                            0                       0
79                      0                            0                       0
80                      0                            0                       0
81                      0                            0                       0
   dietICRR:age:tissueblood dietICRRF:age:tissueblood dietAL:agew50:tissueblood
1                         0                         0                         0
2                         0                         0                         0
3                         0                         0                         0
4                         0                         0                         0
5                         0                         0                         0
6                         0                         0                         0
7                         0                         0                         0
8                         0                         0                         0
9                         0                         0                         0
10                        0                         0                         0
11                        0                         0                         0
12                        0                         0                         0
13                        0                         0                         1
14                        0                         0                         1
15                        0                         0                         1
16                        0                         0                         0
17                        0                         0                         0
18                        0                         0                         0
19                        0                         0                         0
20                        0                         0                         0
21                        0                         0                         0
22                        0                         0                         0
23                        0                         0                         0
24                        0                         0                         0
25                        0                         0                         0
26                        0                         0                         0
27                        0                         0                         0
28                        0                         0                         0
29                        0                         0                         0
30                        0                         0                         0
31                        0                         0                         0
32                        0                         0                         0
33                        0                         0                         0
34                        0                         0                         0
35                        0                         0                         0
36                        0                         0                         0
37                        0                         0                         0
38                        0                         0                         0
39                        0                         0                         0
40                        0                         0                         0
41                        0                         0                         0
42                        0                         0                         0
43                        0                         0                         0
44                        0                         0                         0
45                        0                         0                         0
46                        0                         0                         0
47                        0                         0                         0
48                        0                         0                         0
49                        0                         0                         0
50                        0                         0                         0
51                        0                         0                         0
52                        0                         0                         0
53                        0                         0                         0
54                        0                         0                         0
55                        0                         0                         0
56                        0                         0                         0
57                        0                         0                         0
58                        0                         0                         0
59                        0                         0                         0
60                        0                         0                         0
61                        0                         0                         0
62                        0                         0                         0
63                        0                         0                         0
64                        0                         0                         0
65                        0                         0                         0
66                        0                         0                         0
67                        0                         0                         0
68                        0                         0                         0
69                        0                         0                         0
70                        0                         0                         0
71                        0                         0                         0
72                        0                         0                         0
73                        0                         0                         0
74                        0                         0                         0
75                        0                         0                         0
76                        0                         0                         0
77                        0                         0                         0
78                        0                         0                         0
79                        0                         0                         0
80                        0                         0                         0
81                        0                         0                         0
   dietBASELINE:agew50:tissueblood dietCCR:agew50:tissueblood
1                                0                          0
2                                0                          0
3                                0                          0
4                                0                          0
5                                0                          0
6                                0                          0
7                                0                          0
8                                0                          0
9                                0                          0
10                               0                          0
11                               0                          0
12                               0                          0
13                               0                          0
14                               0                          0
15                               0                          0
16                               0                          1
17                               0                          1
18                               0                          1
19                               0                          0
20                               0                          0
21                               0                          0
22                               0                          0
23                               0                          0
24                               0                          0
25                               0                          0
26                               0                          0
27                               0                          0
28                               0                          0
29                               0                          0
30                               0                          0
31                               0                          0
32                               0                          0
33                               0                          0
34                               0                          0
35                               0                          0
36                               0                          0
37                               0                          0
38                               0                          0
39                               0                          0
40                               0                          0
41                               0                          0
42                               0                          0
43                               0                          0
44                               0                          0
45                               0                          0
46                               0                          0
47                               0                          0
48                               0                          0
49                               0                          0
50                               0                          0
51                               0                          0
52                               0                          0
53                               0                          0
54                               0                          0
55                               0                          0
56                               0                          0
57                               0                          0
58                               0                          0
59                               0                          0
60                               0                          0
61                               0                          0
62                               0                          0
63                               0                          0
64                               0                          0
65                               0                          0
66                               0                          0
67                               0                          0
68                               0                          0
69                               0                          0
70                               0                          0
71                               0                          0
72                               0                          0
73                               0                          0
74                               0                          0
75                               0                          0
76                               0                          0
77                               0                          0
78                               0                          0
79                               0                          0
80                               0                          0
81                               0                          0
   dietICRR:agew50:tissueblood dietICRRF:agew50:tissueblood
1                            0                            0
2                            0                            0
3                            0                            0
4                            0                            0
5                            0                            0
6                            0                            0
7                            0                            0
8                            0                            0
9                            0                            0
10                           0                            0
11                           0                            0
12                           0                            0
13                           0                            0
14                           0                            0
15                           0                            0
16                           0                            0
17                           0                            0
18                           0                            0
19                           1                            0
20                           1                            0
21                           1                            0
22                           0                            1
23                           0                            1
24                           0                            1
25                           0                            0
26                           0                            0
27                           0                            0
28                           0                            0
29                           0                            0
30                           0                            0
31                           0                            0
32                           0                            0
33                           0                            0
34                           0                            0
35                           0                            0
36                           0                            0
37                           0                            0
38                           0                            0
39                           0                            0
40                           0                            0
41                           0                            0
42                           0                            0
43                           0                            0
44                           0                            0
45                           0                            0
46                           0                            0
47                           0                            0
48                           0                            0
49                           0                            0
50                           0                            0
51                           0                            0
52                           0                            0
53                           0                            0
54                           0                            0
55                           0                            0
56                           0                            0
57                           0                            0
58                           0                            0
59                           0                            0
60                           0                            0
61                           0                            0
62                           0                            0
63                           0                            0
64                           0                            0
65                           0                            0
66                           0                            0
67                           0                            0
68                           0                            0
69                           0                            0
70                           0                            0
71                           0                            0
72                           0                            0
73                           0                            0
74                           0                            0
75                           0                            0
76                           0                            0
77                           0                            0
78                           0                            0
79                           0                            0
80                           0                            0
81                           0                            0
   dietAL:ageW50:tissueblood dietBASELINE:ageW50:tissueblood
1                          0                               0
2                          0                               0
3                          0                               0
4                          0                               0
5                          0                               0
6                          0                               0
7                          0                               0
8                          0                               0
9                          0                               0
10                         0                               0
11                         0                               0
12                         0                               0
13                         0                               0
14                         0                               0
15                         0                               0
16                         0                               0
17                         0                               0
18                         0                               0
19                         0                               0
20                         0                               0
21                         0                               0
22                         0                               0
23                         0                               0
24                         0                               0
25                         0                               0
26                         0                               0
27                         0                               0
28                         0                               0
29                         0                               0
30                         0                               0
31                         0                               0
32                         0                               0
33                         0                               0
34                         0                               0
35                         0                               0
36                         0                               0
37                         0                               0
38                         0                               0
39                         0                               0
40                         0                               0
41                         0                               0
42                         0                               0
43                         0                               0
44                         0                               0
45                         0                               0
46                         0                               0
47                         0                               0
48                         0                               0
49                         0                               0
50                         0                               0
51                         0                               0
52                         0                               0
53                         0                               0
54                         0                               0
55                         0                               0
56                         0                               0
57                         0                               0
58                         0                               0
59                         0                               0
60                         0                               0
61                         0                               0
62                         0                               0
63                         0                               0
64                         0                               0
65                         0                               0
66                         0                               0
67                         0                               0
68                         0                               0
69                         0                               0
70                         0                               0
71                         0                               0
72                         0                               0
73                         0                               0
74                         0                               0
75                         0                               0
76                         0                               0
77                         0                               0
78                         0                               0
79                         0                               0
80                         0                               0
81                         0                               0
   dietCCR:ageW50:tissueblood dietICRR:ageW50:tissueblood
1                           0                           0
2                           0                           0
3                           0                           0
4                           0                           0
5                           0                           0
6                           0                           0
7                           0                           0
8                           0                           0
9                           0                           0
10                          0                           0
11                          0                           0
12                          0                           0
13                          0                           0
14                          0                           0
15                          0                           0
16                          0                           0
17                          0                           0
18                          0                           0
19                          0                           0
20                          0                           0
21                          0                           0
22                          0                           0
23                          0                           0
24                          0                           0
25                          0                           0
26                          0                           0
27                          0                           0
28                          0                           0
29                          0                           0
30                          0                           0
31                          0                           0
32                          0                           0
33                          0                           0
34                          0                           0
35                          0                           0
36                          0                           0
37                          0                           0
38                          0                           0
39                          0                           0
40                          0                           0
41                          0                           0
42                          0                           0
43                          0                           0
44                          0                           0
45                          0                           0
46                          0                           0
47                          0                           0
48                          0                           0
49                          0                           0
50                          0                           0
51                          0                           0
52                          0                           0
53                          0                           0
54                          0                           0
55                          0                           0
56                          0                           0
57                          0                           0
58                          0                           0
59                          0                           0
60                          0                           0
61                          0                           0
62                          0                           0
63                          0                           0
64                          0                           0
65                          0                           0
66                          0                           0
67                          0                           0
68                          0                           0
69                          0                           0
70                          0                           0
71                          0                           0
72                          0                           0
73                          0                           0
74                          0                           0
75                          0                           0
76                          0                           0
77                          0                           0
78                          0                           0
79                          0                           0
80                          0                           0
81                          0                           0
   dietICRRF:ageW50:tissueblood dietAL:agew80:tissueblood
1                             0                         0
2                             0                         0
3                             0                         0
4                             0                         0
5                             0                         0
6                             0                         0
7                             0                         0
8                             0                         0
9                             0                         0
10                            0                         0
11                            0                         0
12                            0                         0
13                            0                         0
14                            0                         0
15                            0                         0
16                            0                         0
17                            0                         0
18                            0                         0
19                            0                         0
20                            0                         0
21                            0                         0
22                            0                         0
23                            0                         0
24                            0                         0
25                            0                         1
26                            0                         1
27                            0                         1
28                            0                         0
29                            0                         0
30                            0                         0
31                            0                         0
32                            0                         0
33                            0                         0
34                            0                         0
35                            0                         0
36                            0                         0
37                            0                         0
38                            0                         0
39                            0                         0
40                            0                         0
41                            0                         0
42                            0                         0
43                            0                         0
44                            0                         0
45                            0                         0
46                            0                         0
47                            0                         0
48                            0                         0
49                            0                         0
50                            0                         0
51                            0                         0
52                            0                         0
53                            0                         0
54                            0                         0
55                            0                         0
56                            0                         0
57                            0                         0
58                            0                         0
59                            0                         0
60                            0                         0
61                            0                         0
62                            0                         0
63                            0                         0
64                            0                         0
65                            0                         0
66                            0                         0
67                            0                         0
68                            0                         0
69                            0                         0
70                            0                         0
71                            0                         0
72                            0                         0
73                            0                         0
74                            0                         0
75                            0                         0
76                            0                         0
77                            0                         0
78                            0                         0
79                            0                         0
80                            0                         0
81                            0                         0
   dietBASELINE:agew80:tissueblood dietCCR:agew80:tissueblood
1                                0                          0
2                                0                          0
3                                0                          0
4                                0                          0
5                                0                          0
6                                0                          0
7                                0                          0
8                                0                          0
9                                0                          0
10                               0                          0
11                               0                          0
12                               0                          0
13                               0                          0
14                               0                          0
15                               0                          0
16                               0                          0
17                               0                          0
18                               0                          0
19                               0                          0
20                               0                          0
21                               0                          0
22                               0                          0
23                               0                          0
24                               0                          0
25                               0                          0
26                               0                          0
27                               0                          0
28                               0                          1
29                               0                          1
30                               0                          1
31                               0                          0
32                               0                          0
33                               0                          0
34                               0                          0
35                               0                          0
36                               0                          0
37                               0                          0
38                               0                          0
39                               0                          0
40                               0                          0
41                               0                          0
42                               0                          0
43                               0                          0
44                               0                          0
45                               0                          0
46                               0                          0
47                               0                          0
48                               0                          0
49                               0                          0
50                               0                          0
51                               0                          0
52                               0                          0
53                               0                          0
54                               0                          0
55                               0                          0
56                               0                          0
57                               0                          0
58                               0                          0
59                               0                          0
60                               0                          0
61                               0                          0
62                               0                          0
63                               0                          0
64                               0                          0
65                               0                          0
66                               0                          0
67                               0                          0
68                               0                          0
69                               0                          0
70                               0                          0
71                               0                          0
72                               0                          0
73                               0                          0
74                               0                          0
75                               0                          0
76                               0                          0
77                               0                          0
78                               0                          0
79                               0                          0
80                               0                          0
81                               0                          0
   dietICRR:agew80:tissueblood dietICRRF:agew80:tissueblood dietAL:age:tissuebrain
1                            0                            0                      0
2                            0                            0                      0
3                            0                            0                      0
4                            0                            0                      0
5                            0                            0                      0
6                            0                            0                      0
7                            0                            0                      0
8                            0                            0                      0
9                            0                            0                      0
10                           0                            0                      0
11                           0                            0                      0
12                           0                            0                      0
13                           0                            0                      0
14                           0                            0                      0
15                           0                            0                      0
16                           0                            0                      0
17                           0                            0                      0
18                           0                            0                      0
19                           0                            0                      0
20                           0                            0                      0
21                           0                            0                      0
22                           0                            0                      0
23                           0                            0                      0
24                           0                            0                      0
25                           0                            0                      0
26                           0                            0                      0
27                           0                            0                      0
28                           0                            0                      0
29                           0                            0                      0
30                           0                            0                      0
31                           1                            0                      0
32                           1                            0                      0
33                           1                            0                      0
34                           0                            1                      0
35                           0                            1                      0
36                           0                            1                      0
37                           0                            0                      0
38                           0                            0                      0
39                           0                            0                      0
40                           0                            0                      0
41                           0                            0                      0
42                           0                            0                      0
43                           0                            0                      0
44                           0                            0                      0
45                           0                            0                      0
46                           0                            0                      0
47                           0                            0                      0
48                           0                            0                      0
49                           0                            0                      0
50                           0                            0                      0
51                           0                            0                      0
52                           0                            0                      0
53                           0                            0                      0
54                           0                            0                      0
55                           0                            0                      0
56                           0                            0                      0
57                           0                            0                      0
58                           0                            0                      0
59                           0                            0                      0
60                           0                            0                      0
61                           0                            0                      0
62                           0                            0                      0
63                           0                            0                      0
64                           0                            0                      0
65                           0                            0                      0
66                           0                            0                      0
67                           0                            0                      0
68                           0                            0                      0
69                           0                            0                      0
70                           0                            0                      0
71                           0                            0                      0
72                           0                            0                      0
73                           0                            0                      0
74                           0                            0                      0
75                           0                            0                      0
76                           0                            0                      0
77                           0                            0                      0
78                           0                            0                      0
79                           0                            0                      0
80                           0                            0                      0
81                           0                            0                      0
   dietBASELINE:age:tissuebrain dietCCR:age:tissuebrain dietICRR:age:tissuebrain
1                             0                       0                        0
2                             0                       0                        0
3                             0                       0                        0
4                             0                       0                        0
5                             0                       0                        0
6                             0                       0                        0
7                             0                       0                        0
8                             0                       0                        0
9                             0                       0                        0
10                            0                       0                        0
11                            0                       0                        0
12                            0                       0                        0
13                            0                       0                        0
14                            0                       0                        0
15                            0                       0                        0
16                            0                       0                        0
17                            0                       0                        0
18                            0                       0                        0
19                            0                       0                        0
20                            0                       0                        0
21                            0                       0                        0
22                            0                       0                        0
23                            0                       0                        0
24                            0                       0                        0
25                            0                       0                        0
26                            0                       0                        0
27                            0                       0                        0
28                            0                       0                        0
29                            0                       0                        0
30                            0                       0                        0
31                            0                       0                        0
32                            0                       0                        0
33                            0                       0                        0
34                            0                       0                        0
35                            0                       0                        0
36                            0                       0                        0
37                            0                       0                        0
38                            0                       0                        0
39                            0                       0                        0
40                            0                       0                        0
41                            0                       0                        0
42                            0                       0                        0
43                            0                       0                        0
44                            0                       0                        0
45                            0                       0                        0
46                            0                       0                        0
47                            0                       0                        0
48                            0                       0                        0
49                            0                       0                        0
50                            0                       0                        0
51                            0                       0                        0
52                            0                       0                        0
53                            0                       0                        0
54                            0                       0                        0
55                            0                       0                        0
56                            0                       0                        0
57                            0                       0                        0
58                            0                       0                        0
59                            0                       0                        0
60                            0                       0                        0
61                            1                       0                        0
62                            1                       0                        0
63                            1                       0                        0
64                            0                       0                        0
65                            0                       0                        0
66                            0                       0                        0
67                            0                       0                        0
68                            0                       0                        0
69                            0                       0                        0
70                            0                       0                        0
71                            0                       0                        0
72                            0                       0                        0
73                            0                       0                        0
74                            0                       0                        0
75                            0                       0                        0
76                            0                       0                        0
77                            0                       0                        0
78                            0                       0                        0
79                            0                       0                        0
80                            0                       0                        0
81                            0                       0                        0
   dietICRRF:age:tissuebrain dietAL:agew50:tissuebrain
1                          0                         0
2                          0                         0
3                          0                         0
4                          0                         0
5                          0                         0
6                          0                         0
7                          0                         0
8                          0                         0
9                          0                         0
10                         0                         0
11                         0                         0
12                         0                         0
13                         0                         0
14                         0                         0
15                         0                         0
16                         0                         0
17                         0                         0
18                         0                         0
19                         0                         0
20                         0                         0
21                         0                         0
22                         0                         0
23                         0                         0
24                         0                         0
25                         0                         0
26                         0                         0
27                         0                         0
28                         0                         0
29                         0                         0
30                         0                         0
31                         0                         0
32                         0                         0
33                         0                         0
34                         0                         0
35                         0                         0
36                         0                         0
37                         0                         0
38                         0                         0
39                         0                         0
40                         0                         0
41                         0                         0
42                         0                         0
43                         0                         0
44                         0                         0
45                         0                         0
46                         0                         0
47                         0                         0
48                         0                         0
49                         0                         0
50                         0                         0
51                         0                         0
52                         0                         0
53                         0                         0
54                         0                         0
55                         0                         0
56                         0                         0
57                         0                         0
58                         0                         0
59                         0                         0
60                         0                         0
61                         0                         0
62                         0                         0
63                         0                         0
64                         0                         0
65                         0                         0
66                         0                         0
67                         0                         0
68                         0                         0
69                         0                         0
70                         0                         1
71                         0                         1
72                         0                         1
73                         0                         0
74                         0                         0
75                         0                         0
76                         0                         0
77                         0                         0
78                         0                         0
79                         0                         0
80                         0                         0
81                         0                         0
   dietBASELINE:agew50:tissuebrain dietCCR:agew50:tissuebrain
1                                0                          0
2                                0                          0
3                                0                          0
4                                0                          0
5                                0                          0
6                                0                          0
7                                0                          0
8                                0                          0
9                                0                          0
10                               0                          0
11                               0                          0
12                               0                          0
13                               0                          0
14                               0                          0
15                               0                          0
16                               0                          0
17                               0                          0
18                               0                          0
19                               0                          0
20                               0                          0
21                               0                          0
22                               0                          0
23                               0                          0
24                               0                          0
25                               0                          0
26                               0                          0
27                               0                          0
28                               0                          0
29                               0                          0
30                               0                          0
31                               0                          0
32                               0                          0
33                               0                          0
34                               0                          0
35                               0                          0
36                               0                          0
37                               0                          0
38                               0                          0
39                               0                          0
40                               0                          0
41                               0                          0
42                               0                          0
43                               0                          0
44                               0                          0
45                               0                          0
46                               0                          0
47                               0                          0
48                               0                          0
49                               0                          0
50                               0                          0
51                               0                          0
52                               0                          0
53                               0                          0
54                               0                          0
55                               0                          0
56                               0                          0
57                               0                          0
58                               0                          0
59                               0                          0
60                               0                          0
61                               0                          0
62                               0                          0
63                               0                          0
64                               0                          0
65                               0                          0
66                               0                          0
67                               0                          0
68                               0                          0
69                               0                          0
70                               0                          0
71                               0                          0
72                               0                          0
73                               0                          1
74                               0                          1
75                               0                          1
76                               0                          0
77                               0                          0
78                               0                          0
79                               0                          0
80                               0                          0
81                               0                          0
   dietICRR:agew50:tissuebrain dietICRRF:agew50:tissuebrain
1                            0                            0
2                            0                            0
3                            0                            0
4                            0                            0
5                            0                            0
6                            0                            0
7                            0                            0
8                            0                            0
9                            0                            0
10                           0                            0
11                           0                            0
12                           0                            0
13                           0                            0
14                           0                            0
15                           0                            0
16                           0                            0
17                           0                            0
18                           0                            0
19                           0                            0
20                           0                            0
21                           0                            0
22                           0                            0
23                           0                            0
24                           0                            0
25                           0                            0
26                           0                            0
27                           0                            0
28                           0                            0
29                           0                            0
30                           0                            0
31                           0                            0
32                           0                            0
33                           0                            0
34                           0                            0
35                           0                            0
36                           0                            0
37                           0                            0
38                           0                            0
39                           0                            0
40                           0                            0
41                           0                            0
42                           0                            0
43                           0                            0
44                           0                            0
45                           0                            0
46                           0                            0
47                           0                            0
48                           0                            0
49                           0                            0
50                           0                            0
51                           0                            0
52                           0                            0
53                           0                            0
54                           0                            0
55                           0                            0
56                           0                            0
57                           0                            0
58                           0                            0
59                           0                            0
60                           0                            0
61                           0                            0
62                           0                            0
63                           0                            0
64                           0                            0
65                           0                            0
66                           0                            0
67                           0                            0
68                           0                            0
69                           0                            0
70                           0                            0
71                           0                            0
72                           0                            0
73                           0                            0
74                           0                            0
75                           0                            0
76                           0                            0
77                           1                            0
78                           0                            0
79                           0                            0
80                           0                            0
81                           0                            0
   dietAL:ageW50:tissuebrain dietBASELINE:ageW50:tissuebrain
1                          0                               0
2                          0                               0
3                          0                               0
4                          0                               0
5                          0                               0
6                          0                               0
7                          0                               0
8                          0                               0
9                          0                               0
10                         0                               0
11                         0                               0
12                         0                               0
13                         0                               0
14                         0                               0
15                         0                               0
16                         0                               0
17                         0                               0
18                         0                               0
19                         0                               0
20                         0                               0
21                         0                               0
22                         0                               0
23                         0                               0
24                         0                               0
25                         0                               0
26                         0                               0
27                         0                               0
28                         0                               0
29                         0                               0
30                         0                               0
31                         0                               0
32                         0                               0
33                         0                               0
34                         0                               0
35                         0                               0
36                         0                               0
37                         0                               0
38                         0                               0
39                         0                               0
40                         0                               0
41                         0                               0
42                         0                               0
43                         0                               0
44                         0                               0
45                         0                               0
46                         0                               0
47                         0                               0
48                         0                               0
49                         0                               0
50                         0                               0
51                         0                               0
52                         0                               0
53                         0                               0
54                         0                               0
55                         0                               0
56                         0                               0
57                         0                               0
58                         0                               0
59                         0                               0
60                         0                               0
61                         0                               0
62                         0                               0
63                         0                               0
64                         0                               0
65                         0                               0
66                         0                               0
67                         0                               0
68                         0                               0
69                         0                               0
70                         0                               0
71                         0                               0
72                         0                               0
73                         0                               0
74                         0                               0
75                         0                               0
76                         0                               0
77                         0                               0
78                         0                               0
79                         0                               0
80                         0                               0
81                         0                               0
   dietCCR:ageW50:tissuebrain dietICRR:ageW50:tissuebrain
1                           0                           0
2                           0                           0
3                           0                           0
4                           0                           0
5                           0                           0
6                           0                           0
7                           0                           0
8                           0                           0
9                           0                           0
10                          0                           0
11                          0                           0
12                          0                           0
13                          0                           0
14                          0                           0
15                          0                           0
16                          0                           0
17                          0                           0
18                          0                           0
19                          0                           0
20                          0                           0
21                          0                           0
22                          0                           0
23                          0                           0
24                          0                           0
25                          0                           0
26                          0                           0
27                          0                           0
28                          0                           0
29                          0                           0
30                          0                           0
31                          0                           0
32                          0                           0
33                          0                           0
34                          0                           0
35                          0                           0
36                          0                           0
37                          0                           0
38                          0                           0
39                          0                           0
40                          0                           0
41                          0                           0
42                          0                           0
43                          0                           0
44                          0                           0
45                          0                           0
46                          0                           0
47                          0                           0
48                          0                           0
49                          0                           0
50                          0                           0
51                          0                           0
52                          0                           0
53                          0                           0
54                          0                           0
55                          0                           0
56                          0                           0
57                          0                           0
58                          0                           0
59                          0                           0
60                          0                           0
61                          0                           0
62                          0                           0
63                          0                           0
64                          0                           0
65                          0                           0
66                          0                           0
67                          0                           0
68                          0                           0
69                          0                           0
70                          0                           0
71                          0                           0
72                          0                           0
73                          0                           0
74                          0                           0
75                          0                           0
76                          0                           1
77                          0                           0
78                          0                           1
79                          0                           0
80                          0                           0
81                          0                           0
   dietICRRF:ageW50:tissuebrain dietAL:agew80:tissuebrain
1                             0                         1
2                             0                         1
3                             0                         1
4                             0                         0
5                             0                         0
6                             0                         0
7                             0                         0
8                             0                         0
9                             0                         0
10                            0                         0
11                            0                         0
12                            0                         0
13                            0                         0
14                            0                         0
15                            0                         0
16                            0                         0
17                            0                         0
18                            0                         0
19                            0                         0
20                            0                         0
21                            0                         0
22                            0                         0
23                            0                         0
24                            0                         0
25                            0                         0
26                            0                         0
27                            0                         0
28                            0                         0
29                            0                         0
30                            0                         0
31                            0                         0
32                            0                         0
33                            0                         0
34                            0                         0
35                            0                         0
36                            0                         0
37                            0                         0
38                            0                         0
39                            0                         0
40                            0                         0
41                            0                         0
42                            0                         0
43                            0                         0
44                            0                         0
45                            0                         0
46                            0                         0
47                            0                         0
48                            0                         0
49                            0                         0
50                            0                         0
51                            0                         0
52                            0                         0
53                            0                         0
54                            0                         0
55                            0                         0
56                            0                         0
57                            0                         0
58                            0                         0
59                            0                         0
60                            0                         0
61                            0                         0
62                            0                         0
63                            0                         0
64                            0                         0
65                            0                         0
66                            0                         0
67                            0                         0
68                            0                         0
69                            0                         0
70                            0                         0
71                            0                         0
72                            0                         0
73                            0                         0
74                            0                         0
75                            0                         0
76                            0                         0
77                            0                         0
78                            0                         0
79                            1                         0
80                            1                         0
81                            1                         0
   dietBASELINE:agew80:tissuebrain dietCCR:agew80:tissuebrain
1                                0                          0
2                                0                          0
3                                0                          0
4                                0                          1
5                                0                          1
6                                0                          1
7                                0                          0
8                                0                          0
9                                0                          0
10                               0                          0
11                               0                          0
12                               0                          0
13                               0                          0
14                               0                          0
15                               0                          0
16                               0                          0
17                               0                          0
18                               0                          0
19                               0                          0
20                               0                          0
21                               0                          0
22                               0                          0
23                               0                          0
24                               0                          0
25                               0                          0
26                               0                          0
27                               0                          0
28                               0                          0
29                               0                          0
30                               0                          0
31                               0                          0
32                               0                          0
33                               0                          0
34                               0                          0
35                               0                          0
36                               0                          0
37                               0                          0
38                               0                          0
39                               0                          0
40                               0                          0
41                               0                          0
42                               0                          0
43                               0                          0
44                               0                          0
45                               0                          0
46                               0                          0
47                               0                          0
48                               0                          0
49                               0                          0
50                               0                          0
51                               0                          0
52                               0                          0
53                               0                          0
54                               0                          0
55                               0                          0
56                               0                          0
57                               0                          0
58                               0                          0
59                               0                          0
60                               0                          0
61                               0                          0
62                               0                          0
63                               0                          0
64                               0                          0
65                               0                          0
66                               0                          0
67                               0                          0
68                               0                          0
69                               0                          0
70                               0                          0
71                               0                          0
72                               0                          0
73                               0                          0
74                               0                          0
75                               0                          0
76                               0                          0
77                               0                          0
78                               0                          0
79                               0                          0
80                               0                          0
81                               0                          0
   dietICRR:agew80:tissuebrain dietICRRF:agew80:tissuebrain dietAL:age:tissuemfp
1                            0                            0                    0
2                            0                            0                    0
3                            0                            0                    0
4                            0                            0                    0
5                            0                            0                    0
6                            0                            0                    0
7                            1                            0                    0
8                            1                            0                    0
9                            1                            0                    0
10                           0                            1                    0
11                           0                            1                    0
12                           0                            1                    0
13                           0                            0                    0
14                           0                            0                    0
15                           0                            0                    0
16                           0                            0                    0
17                           0                            0                    0
18                           0                            0                    0
19                           0                            0                    0
20                           0                            0                    0
21                           0                            0                    0
22                           0                            0                    0
23                           0                            0                    0
24                           0                            0                    0
25                           0                            0                    0
26                           0                            0                    0
27                           0                            0                    0
28                           0                            0                    0
29                           0                            0                    0
30                           0                            0                    0
31                           0                            0                    0
32                           0                            0                    0
33                           0                            0                    0
34                           0                            0                    0
35                           0                            0                    0
36                           0                            0                    0
37                           0                            0                    0
38                           0                            0                    0
39                           0                            0                    0
40                           0                            0                    0
41                           0                            0                    0
42                           0                            0                    0
43                           0                            0                    0
44                           0                            0                    0
45                           0                            0                    0
46                           0                            0                    0
47                           0                            0                    0
48                           0                            0                    0
49                           0                            0                    0
50                           0                            0                    0
51                           0                            0                    0
52                           0                            0                    0
53                           0                            0                    0
54                           0                            0                    0
55                           0                            0                    0
56                           0                            0                    0
57                           0                            0                    0
58                           0                            0                    0
59                           0                            0                    0
60                           0                            0                    0
61                           0                            0                    0
62                           0                            0                    0
63                           0                            0                    0
64                           0                            0                    0
65                           0                            0                    0
66                           0                            0                    0
67                           0                            0                    0
68                           0                            0                    0
69                           0                            0                    0
70                           0                            0                    0
71                           0                            0                    0
72                           0                            0                    0
73                           0                            0                    0
74                           0                            0                    0
75                           0                            0                    0
76                           0                            0                    0
77                           0                            0                    0
78                           0                            0                    0
79                           0                            0                    0
80                           0                            0                    0
81                           0                            0                    0
   dietBASELINE:age:tissuemfp dietCCR:age:tissuemfp dietICRR:age:tissuemfp
1                           0                     0                      0
2                           0                     0                      0
3                           0                     0                      0
4                           0                     0                      0
5                           0                     0                      0
6                           0                     0                      0
7                           0                     0                      0
8                           0                     0                      0
9                           0                     0                      0
10                          0                     0                      0
11                          0                     0                      0
12                          0                     0                      0
13                          0                     0                      0
14                          0                     0                      0
15                          0                     0                      0
16                          0                     0                      0
17                          0                     0                      0
18                          0                     0                      0
19                          0                     0                      0
20                          0                     0                      0
21                          0                     0                      0
22                          0                     0                      0
23                          0                     0                      0
24                          0                     0                      0
25                          0                     0                      0
26                          0                     0                      0
27                          0                     0                      0
28                          0                     0                      0
29                          0                     0                      0
30                          0                     0                      0
31                          0                     0                      0
32                          0                     0                      0
33                          0                     0                      0
34                          0                     0                      0
35                          0                     0                      0
36                          0                     0                      0
37                          0                     0                      0
38                          0                     0                      0
39                          0                     0                      0
40                          0                     0                      0
41                          0                     0                      0
42                          0                     0                      0
43                          0                     0                      0
44                          0                     0                      0
45                          0                     0                      0
46                          0                     0                      0
47                          0                     0                      0
48                          0                     0                      0
49                          0                     0                      0
50                          0                     0                      0
51                          0                     0                      0
52                          0                     0                      0
53                          0                     0                      0
54                          0                     0                      0
55                          0                     0                      0
56                          0                     0                      0
57                          0                     0                      0
58                          0                     0                      0
59                          0                     0                      0
60                          0                     0                      0
61                          0                     0                      0
62                          0                     0                      0
63                          0                     0                      0
64                          0                     0                      0
65                          0                     0                      0
66                          0                     0                      0
67                          1                     0                      0
68                          1                     0                      0
69                          1                     0                      0
70                          0                     0                      0
71                          0                     0                      0
72                          0                     0                      0
73                          0                     0                      0
74                          0                     0                      0
75                          0                     0                      0
76                          0                     0                      0
77                          0                     0                      0
78                          0                     0                      0
79                          0                     0                      0
80                          0                     0                      0
81                          0                     0                      0
   dietICRRF:age:tissuemfp dietAL:agew50:tissuemfp dietBASELINE:agew50:tissuemfp
1                        0                       0                             0
2                        0                       0                             0
3                        0                       0                             0
4                        0                       0                             0
5                        0                       0                             0
6                        0                       0                             0
7                        0                       0                             0
8                        0                       0                             0
9                        0                       0                             0
10                       0                       0                             0
11                       0                       0                             0
12                       0                       0                             0
13                       0                       0                             0
14                       0                       0                             0
15                       0                       0                             0
16                       0                       0                             0
17                       0                       0                             0
18                       0                       0                             0
19                       0                       0                             0
20                       0                       0                             0
21                       0                       0                             0
22                       0                       0                             0
23                       0                       0                             0
24                       0                       0                             0
25                       0                       0                             0
26                       0                       0                             0
27                       0                       0                             0
28                       0                       0                             0
29                       0                       0                             0
30                       0                       0                             0
31                       0                       0                             0
32                       0                       0                             0
33                       0                       0                             0
34                       0                       0                             0
35                       0                       0                             0
36                       0                       0                             0
37                       0                       1                             0
38                       0                       1                             0
39                       0                       1                             0
40                       0                       0                             0
41                       0                       0                             0
42                       0                       0                             0
43                       0                       0                             0
44                       0                       0                             0
45                       0                       0                             0
46                       0                       0                             0
47                       0                       0                             0
48                       0                       0                             0
49                       0                       0                             0
50                       0                       0                             0
51                       0                       0                             0
52                       0                       0                             0
53                       0                       0                             0
54                       0                       0                             0
55                       0                       0                             0
56                       0                       0                             0
57                       0                       0                             0
58                       0                       0                             0
59                       0                       0                             0
60                       0                       0                             0
61                       0                       0                             0
62                       0                       0                             0
63                       0                       0                             0
64                       0                       0                             0
65                       0                       0                             0
66                       0                       0                             0
67                       0                       0                             0
68                       0                       0                             0
69                       0                       0                             0
70                       0                       0                             0
71                       0                       0                             0
72                       0                       0                             0
73                       0                       0                             0
74                       0                       0                             0
75                       0                       0                             0
76                       0                       0                             0
77                       0                       0                             0
78                       0                       0                             0
79                       0                       0                             0
80                       0                       0                             0
81                       0                       0                             0
   dietCCR:agew50:tissuemfp dietICRR:agew50:tissuemfp dietICRRF:agew50:tissuemfp
1                         0                         0                          0
2                         0                         0                          0
3                         0                         0                          0
4                         0                         0                          0
5                         0                         0                          0
6                         0                         0                          0
7                         0                         0                          0
8                         0                         0                          0
9                         0                         0                          0
10                        0                         0                          0
11                        0                         0                          0
12                        0                         0                          0
13                        0                         0                          0
14                        0                         0                          0
15                        0                         0                          0
16                        0                         0                          0
17                        0                         0                          0
18                        0                         0                          0
19                        0                         0                          0
20                        0                         0                          0
21                        0                         0                          0
22                        0                         0                          0
23                        0                         0                          0
24                        0                         0                          0
25                        0                         0                          0
26                        0                         0                          0
27                        0                         0                          0
28                        0                         0                          0
29                        0                         0                          0
30                        0                         0                          0
31                        0                         0                          0
32                        0                         0                          0
33                        0                         0                          0
34                        0                         0                          0
35                        0                         0                          0
36                        0                         0                          0
37                        0                         0                          0
38                        0                         0                          0
39                        0                         0                          0
40                        1                         0                          0
41                        1                         0                          0
42                        1                         0                          0
43                        0                         1                          0
44                        0                         1                          0
45                        0                         1                          0
46                        0                         0                          1
47                        0                         0                          1
48                        0                         0                          1
49                        0                         0                          0
50                        0                         0                          0
51                        0                         0                          0
52                        0                         0                          0
53                        0                         0                          0
54                        0                         0                          0
55                        0                         0                          0
56                        0                         0                          0
57                        0                         0                          0
58                        0                         0                          0
59                        0                         0                          0
60                        0                         0                          0
61                        0                         0                          0
62                        0                         0                          0
63                        0                         0                          0
64                        0                         0                          0
65                        0                         0                          0
66                        0                         0                          0
67                        0                         0                          0
68                        0                         0                          0
69                        0                         0                          0
70                        0                         0                          0
71                        0                         0                          0
72                        0                         0                          0
73                        0                         0                          0
74                        0                         0                          0
75                        0                         0                          0
76                        0                         0                          0
77                        0                         0                          0
78                        0                         0                          0
79                        0                         0                          0
80                        0                         0                          0
81                        0                         0                          0
   dietAL:ageW50:tissuemfp dietBASELINE:ageW50:tissuemfp dietCCR:ageW50:tissuemfp
1                        0                             0                        0
2                        0                             0                        0
3                        0                             0                        0
4                        0                             0                        0
5                        0                             0                        0
6                        0                             0                        0
7                        0                             0                        0
8                        0                             0                        0
9                        0                             0                        0
10                       0                             0                        0
11                       0                             0                        0
12                       0                             0                        0
13                       0                             0                        0
14                       0                             0                        0
15                       0                             0                        0
16                       0                             0                        0
17                       0                             0                        0
18                       0                             0                        0
19                       0                             0                        0
20                       0                             0                        0
21                       0                             0                        0
22                       0                             0                        0
23                       0                             0                        0
24                       0                             0                        0
25                       0                             0                        0
26                       0                             0                        0
27                       0                             0                        0
28                       0                             0                        0
29                       0                             0                        0
30                       0                             0                        0
31                       0                             0                        0
32                       0                             0                        0
33                       0                             0                        0
34                       0                             0                        0
35                       0                             0                        0
36                       0                             0                        0
37                       0                             0                        0
38                       0                             0                        0
39                       0                             0                        0
40                       0                             0                        0
41                       0                             0                        0
42                       0                             0                        0
43                       0                             0                        0
44                       0                             0                        0
45                       0                             0                        0
46                       0                             0                        0
47                       0                             0                        0
48                       0                             0                        0
49                       0                             0                        0
50                       0                             0                        0
51                       0                             0                        0
52                       0                             0                        0
53                       0                             0                        0
54                       0                             0                        0
55                       0                             0                        0
56                       0                             0                        0
57                       0                             0                        0
58                       0                             0                        0
59                       0                             0                        0
60                       0                             0                        0
61                       0                             0                        0
62                       0                             0                        0
63                       0                             0                        0
64                       0                             0                        0
65                       0                             0                        0
66                       0                             0                        0
67                       0                             0                        0
68                       0                             0                        0
69                       0                             0                        0
70                       0                             0                        0
71                       0                             0                        0
72                       0                             0                        0
73                       0                             0                        0
74                       0                             0                        0
75                       0                             0                        0
76                       0                             0                        0
77                       0                             0                        0
78                       0                             0                        0
79                       0                             0                        0
80                       0                             0                        0
81                       0                             0                        0
   dietICRR:ageW50:tissuemfp dietICRRF:ageW50:tissuemfp dietAL:agew80:tissuemfp
1                          0                          0                       0
2                          0                          0                       0
3                          0                          0                       0
4                          0                          0                       0
5                          0                          0                       0
6                          0                          0                       0
7                          0                          0                       0
8                          0                          0                       0
9                          0                          0                       0
10                         0                          0                       0
11                         0                          0                       0
12                         0                          0                       0
13                         0                          0                       0
14                         0                          0                       0
15                         0                          0                       0
16                         0                          0                       0
17                         0                          0                       0
18                         0                          0                       0
19                         0                          0                       0
20                         0                          0                       0
21                         0                          0                       0
22                         0                          0                       0
23                         0                          0                       0
24                         0                          0                       0
25                         0                          0                       0
26                         0                          0                       0
27                         0                          0                       0
28                         0                          0                       0
29                         0                          0                       0
30                         0                          0                       0
31                         0                          0                       0
32                         0                          0                       0
33                         0                          0                       0
34                         0                          0                       0
35                         0                          0                       0
36                         0                          0                       0
37                         0                          0                       0
38                         0                          0                       0
39                         0                          0                       0
40                         0                          0                       0
41                         0                          0                       0
42                         0                          0                       0
43                         0                          0                       0
44                         0                          0                       0
45                         0                          0                       0
46                         0                          0                       0
47                         0                          0                       0
48                         0                          0                       0
49                         0                          0                       1
50                         0                          0                       1
51                         0                          0                       1
52                         0                          0                       0
53                         0                          0                       0
54                         0                          0                       0
55                         0                          0                       0
56                         0                          0                       0
57                         0                          0                       0
58                         0                          0                       0
59                         0                          0                       0
60                         0                          0                       0
61                         0                          0                       0
62                         0                          0                       0
63                         0                          0                       0
64                         0                          0                       0
65                         0                          0                       0
66                         0                          0                       0
67                         0                          0                       0
68                         0                          0                       0
69                         0                          0                       0
70                         0                          0                       0
71                         0                          0                       0
72                         0                          0                       0
73                         0                          0                       0
74                         0                          0                       0
75                         0                          0                       0
76                         0                          0                       0
77                         0                          0                       0
78                         0                          0                       0
79                         0                          0                       0
80                         0                          0                       0
81                         0                          0                       0
   dietBASELINE:agew80:tissuemfp dietCCR:agew80:tissuemfp dietICRR:agew80:tissuemfp
1                              0                        0                         0
2                              0                        0                         0
3                              0                        0                         0
4                              0                        0                         0
5                              0                        0                         0
6                              0                        0                         0
7                              0                        0                         0
8                              0                        0                         0
9                              0                        0                         0
10                             0                        0                         0
11                             0                        0                         0
12                             0                        0                         0
13                             0                        0                         0
14                             0                        0                         0
15                             0                        0                         0
16                             0                        0                         0
17                             0                        0                         0
18                             0                        0                         0
19                             0                        0                         0
20                             0                        0                         0
21                             0                        0                         0
22                             0                        0                         0
23                             0                        0                         0
24                             0                        0                         0
25                             0                        0                         0
26                             0                        0                         0
27                             0                        0                         0
28                             0                        0                         0
29                             0                        0                         0
30                             0                        0                         0
31                             0                        0                         0
32                             0                        0                         0
33                             0                        0                         0
34                             0                        0                         0
35                             0                        0                         0
36                             0                        0                         0
37                             0                        0                         0
38                             0                        0                         0
39                             0                        0                         0
40                             0                        0                         0
41                             0                        0                         0
42                             0                        0                         0
43                             0                        0                         0
44                             0                        0                         0
45                             0                        0                         0
46                             0                        0                         0
47                             0                        0                         0
48                             0                        0                         0
49                             0                        0                         0
50                             0                        0                         0
51                             0                        0                         0
52                             0                        1                         0
53                             0                        1                         0
54                             0                        1                         0
55                             0                        0                         1
56                             0                        0                         1
57                             0                        0                         1
58                             0                        0                         0
59                             0                        0                         0
60                             0                        0                         0
61                             0                        0                         0
62                             0                        0                         0
63                             0                        0                         0
64                             0                        0                         0
65                             0                        0                         0
66                             0                        0                         0
67                             0                        0                         0
68                             0                        0                         0
69                             0                        0                         0
70                             0                        0                         0
71                             0                        0                         0
72                             0                        0                         0
73                             0                        0                         0
74                             0                        0                         0
75                             0                        0                         0
76                             0                        0                         0
77                             0                        0                         0
78                             0                        0                         0
79                             0                        0                         0
80                             0                        0                         0
81                             0                        0                         0
   dietICRRF:agew80:tissuemfp
1                           0
2                           0
3                           0
4                           0
5                           0
6                           0
7                           0
8                           0
9                           0
10                          0
11                          0
12                          0
13                          0
14                          0
15                          0
16                          0
17                          0
18                          0
19                          0
20                          0
21                          0
22                          0
23                          0
24                          0
25                          0
26                          0
27                          0
28                          0
29                          0
30                          0
31                          0
32                          0
33                          0
34                          0
35                          0
36                          0
37                          0
38                          0
39                          0
40                          0
41                          0
42                          0
43                          0
44                          0
45                          0
46                          0
47                          0
48                          0
49                          0
50                          0
51                          0
52                          0
53                          0
54                          0
55                          0
56                          0
57                          0
58                          1
59                          1
60                          1
61                          0
62                          0
63                          0
64                          0
65                          0
66                          0
67                          0
68                          0
69                          0
70                          0
71                          0
72                          0
73                          0
74                          0
75                          0
76                          0
77                          0
78                          0
79                          0
80                          0
81                          0
attr(,"assign")
 [1] 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
[41] 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
attr(,"contrasts")
attr(,"contrasts")$diet
[1] "contr.treatment"

attr(,"contrasts")$age
[1] "contr.treatment"

attr(,"contrasts")$tissue
[1] "contr.treatment"

Rename column names

design
   BASELINE.10.Blood AL.49.Blood CCR.49.Blood ICRR.49.Blood ICRRF.49.Blood
1                  0           0            0             0              0
2                  0           0            0             0              0
3                  0           0            0             0              0
4                  0           0            0             0              0
5                  0           0            0             0              0
6                  0           0            0             0              0
7                  0           0            0             0              0
8                  0           0            0             0              0
9                  0           0            0             0              0
10                 0           0            0             0              0
11                 0           0            0             0              0
12                 0           0            0             0              0
13                 0           1            0             0              0
14                 0           1            0             0              0
15                 0           1            0             0              0
16                 0           0            1             0              0
17                 0           0            1             0              0
18                 0           0            1             0              0
19                 0           0            0             1              0
20                 0           0            0             1              0
21                 0           0            0             1              0
22                 0           0            0             0              1
23                 0           0            0             0              1
24                 0           0            0             0              1
25                 0           0            0             0              0
26                 0           0            0             0              0
27                 0           0            0             0              0
28                 0           0            0             0              0
29                 0           0            0             0              0
30                 0           0            0             0              0
31                 0           0            0             0              0
32                 0           0            0             0              0
33                 0           0            0             0              0
34                 0           0            0             0              0
35                 0           0            0             0              0
36                 0           0            0             0              0
37                 0           0            0             0              0
38                 0           0            0             0              0
39                 0           0            0             0              0
40                 0           0            0             0              0
41                 0           0            0             0              0
42                 0           0            0             0              0
43                 0           0            0             0              0
44                 0           0            0             0              0
45                 0           0            0             0              0
46                 0           0            0             0              0
47                 0           0            0             0              0
48                 0           0            0             0              0
49                 0           0            0             0              0
50                 0           0            0             0              0
51                 0           0            0             0              0
52                 0           0            0             0              0
53                 0           0            0             0              0
54                 0           0            0             0              0
55                 0           0            0             0              0
56                 0           0            0             0              0
57                 0           0            0             0              0
58                 0           0            0             0              0
59                 0           0            0             0              0
60                 0           0            0             0              0
61                 0           0            0             0              0
62                 0           0            0             0              0
63                 0           0            0             0              0
64                 1           0            0             0              0
65                 1           0            0             0              0
66                 1           0            0             0              0
67                 0           0            0             0              0
68                 0           0            0             0              0
69                 0           0            0             0              0
70                 0           0            0             0              0
71                 0           0            0             0              0
72                 0           0            0             0              0
73                 0           0            0             0              0
74                 0           0            0             0              0
75                 0           0            0             0              0
76                 0           0            0             0              0
77                 0           0            0             0              0
78                 0           0            0             0              0
79                 0           0            0             0              0
80                 0           0            0             0              0
81                 0           0            0             0              0
   AL.81.Blood CCR.81.Blood ICRR.81.Blood ICRRF.81.Blood BASELINE.10.Brain
1            0            0             0              0                 0
2            0            0             0              0                 0
3            0            0             0              0                 0
4            0            0             0              0                 0
5            0            0             0              0                 0
6            0            0             0              0                 0
7            0            0             0              0                 0
8            0            0             0              0                 0
9            0            0             0              0                 0
10           0            0             0              0                 0
11           0            0             0              0                 0
12           0            0             0              0                 0
13           0            0             0              0                 0
14           0            0             0              0                 0
15           0            0             0              0                 0
16           0            0             0              0                 0
17           0            0             0              0                 0
18           0            0             0              0                 0
19           0            0             0              0                 0
20           0            0             0              0                 0
21           0            0             0              0                 0
22           0            0             0              0                 0
23           0            0             0              0                 0
24           0            0             0              0                 0
25           1            0             0              0                 0
26           1            0             0              0                 0
27           1            0             0              0                 0
28           0            1             0              0                 0
29           0            1             0              0                 0
30           0            1             0              0                 0
31           0            0             1              0                 0
32           0            0             1              0                 0
33           0            0             1              0                 0
34           0            0             0              1                 0
35           0            0             0              1                 0
36           0            0             0              1                 0
37           0            0             0              0                 0
38           0            0             0              0                 0
39           0            0             0              0                 0
40           0            0             0              0                 0
41           0            0             0              0                 0
42           0            0             0              0                 0
43           0            0             0              0                 0
44           0            0             0              0                 0
45           0            0             0              0                 0
46           0            0             0              0                 0
47           0            0             0              0                 0
48           0            0             0              0                 0
49           0            0             0              0                 0
50           0            0             0              0                 0
51           0            0             0              0                 0
52           0            0             0              0                 0
53           0            0             0              0                 0
54           0            0             0              0                 0
55           0            0             0              0                 0
56           0            0             0              0                 0
57           0            0             0              0                 0
58           0            0             0              0                 0
59           0            0             0              0                 0
60           0            0             0              0                 0
61           0            0             0              0                 1
62           0            0             0              0                 1
63           0            0             0              0                 1
64           0            0             0              0                 0
65           0            0             0              0                 0
66           0            0             0              0                 0
67           0            0             0              0                 0
68           0            0             0              0                 0
69           0            0             0              0                 0
70           0            0             0              0                 0
71           0            0             0              0                 0
72           0            0             0              0                 0
73           0            0             0              0                 0
74           0            0             0              0                 0
75           0            0             0              0                 0
76           0            0             0              0                 0
77           0            0             0              0                 0
78           0            0             0              0                 0
79           0            0             0              0                 0
80           0            0             0              0                 0
81           0            0             0              0                 0
   AL.49.Brain CCR.49.Brain ICRR.49.Brain ICRRF.49.Brain AL.81.Brain CCR.81.Brain
1            0            0             0              0           1            0
2            0            0             0              0           1            0
3            0            0             0              0           1            0
4            0            0             0              0           0            1
5            0            0             0              0           0            1
6            0            0             0              0           0            1
7            0            0             0              0           0            0
8            0            0             0              0           0            0
9            0            0             0              0           0            0
10           0            0             0              0           0            0
11           0            0             0              0           0            0
12           0            0             0              0           0            0
13           0            0             0              0           0            0
14           0            0             0              0           0            0
15           0            0             0              0           0            0
16           0            0             0              0           0            0
17           0            0             0              0           0            0
18           0            0             0              0           0            0
19           0            0             0              0           0            0
20           0            0             0              0           0            0
21           0            0             0              0           0            0
22           0            0             0              0           0            0
23           0            0             0              0           0            0
24           0            0             0              0           0            0
25           0            0             0              0           0            0
26           0            0             0              0           0            0
27           0            0             0              0           0            0
28           0            0             0              0           0            0
29           0            0             0              0           0            0
30           0            0             0              0           0            0
31           0            0             0              0           0            0
32           0            0             0              0           0            0
33           0            0             0              0           0            0
34           0            0             0              0           0            0
35           0            0             0              0           0            0
36           0            0             0              0           0            0
37           0            0             0              0           0            0
38           0            0             0              0           0            0
39           0            0             0              0           0            0
40           0            0             0              0           0            0
41           0            0             0              0           0            0
42           0            0             0              0           0            0
43           0            0             0              0           0            0
44           0            0             0              0           0            0
45           0            0             0              0           0            0
46           0            0             0              0           0            0
47           0            0             0              0           0            0
48           0            0             0              0           0            0
49           0            0             0              0           0            0
50           0            0             0              0           0            0
51           0            0             0              0           0            0
52           0            0             0              0           0            0
53           0            0             0              0           0            0
54           0            0             0              0           0            0
55           0            0             0              0           0            0
56           0            0             0              0           0            0
57           0            0             0              0           0            0
58           0            0             0              0           0            0
59           0            0             0              0           0            0
60           0            0             0              0           0            0
61           0            0             0              0           0            0
62           0            0             0              0           0            0
63           0            0             0              0           0            0
64           0            0             0              0           0            0
65           0            0             0              0           0            0
66           0            0             0              0           0            0
67           0            0             0              0           0            0
68           0            0             0              0           0            0
69           0            0             0              0           0            0
70           1            0             0              0           0            0
71           1            0             0              0           0            0
72           1            0             0              0           0            0
73           0            1             0              0           0            0
74           0            1             0              0           0            0
75           0            1             0              0           0            0
76           0            0             1              0           0            0
77           0            0             1              0           0            0
78           0            0             1              0           0            0
79           0            0             0              1           0            0
80           0            0             0              1           0            0
81           0            0             0              1           0            0
   ICRR.81.Brain ICRRF.81.Brain BASELINE.10.MFP AL.49.MFP CCR.49.MFP ICRR.49.MFP
1              0              0               0         0          0           0
2              0              0               0         0          0           0
3              0              0               0         0          0           0
4              0              0               0         0          0           0
5              0              0               0         0          0           0
6              0              0               0         0          0           0
7              1              0               0         0          0           0
8              1              0               0         0          0           0
9              1              0               0         0          0           0
10             0              1               0         0          0           0
11             0              1               0         0          0           0
12             0              1               0         0          0           0
13             0              0               0         0          0           0
14             0              0               0         0          0           0
15             0              0               0         0          0           0
16             0              0               0         0          0           0
17             0              0               0         0          0           0
18             0              0               0         0          0           0
19             0              0               0         0          0           0
20             0              0               0         0          0           0
21             0              0               0         0          0           0
22             0              0               0         0          0           0
23             0              0               0         0          0           0
24             0              0               0         0          0           0
25             0              0               0         0          0           0
26             0              0               0         0          0           0
27             0              0               0         0          0           0
28             0              0               0         0          0           0
29             0              0               0         0          0           0
30             0              0               0         0          0           0
31             0              0               0         0          0           0
32             0              0               0         0          0           0
33             0              0               0         0          0           0
34             0              0               0         0          0           0
35             0              0               0         0          0           0
36             0              0               0         0          0           0
37             0              0               0         1          0           0
38             0              0               0         1          0           0
39             0              0               0         1          0           0
40             0              0               0         0          1           0
41             0              0               0         0          1           0
42             0              0               0         0          1           0
43             0              0               0         0          0           1
44             0              0               0         0          0           1
45             0              0               0         0          0           1
46             0              0               0         0          0           0
47             0              0               0         0          0           0
48             0              0               0         0          0           0
49             0              0               0         0          0           0
50             0              0               0         0          0           0
51             0              0               0         0          0           0
52             0              0               0         0          0           0
53             0              0               0         0          0           0
54             0              0               0         0          0           0
55             0              0               0         0          0           0
56             0              0               0         0          0           0
57             0              0               0         0          0           0
58             0              0               0         0          0           0
59             0              0               0         0          0           0
60             0              0               0         0          0           0
61             0              0               0         0          0           0
62             0              0               0         0          0           0
63             0              0               0         0          0           0
64             0              0               0         0          0           0
65             0              0               0         0          0           0
66             0              0               0         0          0           0
67             0              0               1         0          0           0
68             0              0               1         0          0           0
69             0              0               1         0          0           0
70             0              0               0         0          0           0
71             0              0               0         0          0           0
72             0              0               0         0          0           0
73             0              0               0         0          0           0
74             0              0               0         0          0           0
75             0              0               0         0          0           0
76             0              0               0         0          0           0
77             0              0               0         0          0           0
78             0              0               0         0          0           0
79             0              0               0         0          0           0
80             0              0               0         0          0           0
81             0              0               0         0          0           0
   ICRRF.49.MFP AL.81.MFP CCR.81.MFP ICRR.81.MFP ICRRF.81.MFP
1             0         0          0           0            0
2             0         0          0           0            0
3             0         0          0           0            0
4             0         0          0           0            0
5             0         0          0           0            0
6             0         0          0           0            0
7             0         0          0           0            0
8             0         0          0           0            0
9             0         0          0           0            0
10            0         0          0           0            0
11            0         0          0           0            0
12            0         0          0           0            0
13            0         0          0           0            0
14            0         0          0           0            0
15            0         0          0           0            0
16            0         0          0           0            0
17            0         0          0           0            0
18            0         0          0           0            0
19            0         0          0           0            0
20            0         0          0           0            0
21            0         0          0           0            0
22            0         0          0           0            0
23            0         0          0           0            0
24            0         0          0           0            0
25            0         0          0           0            0
26            0         0          0           0            0
27            0         0          0           0            0
28            0         0          0           0            0
29            0         0          0           0            0
30            0         0          0           0            0
31            0         0          0           0            0
32            0         0          0           0            0
33            0         0          0           0            0
34            0         0          0           0            0
35            0         0          0           0            0
36            0         0          0           0            0
37            0         0          0           0            0
38            0         0          0           0            0
39            0         0          0           0            0
40            0         0          0           0            0
41            0         0          0           0            0
42            0         0          0           0            0
43            0         0          0           0            0
44            0         0          0           0            0
45            0         0          0           0            0
46            1         0          0           0            0
47            1         0          0           0            0
48            1         0          0           0            0
49            0         1          0           0            0
50            0         1          0           0            0
51            0         1          0           0            0
52            0         0          1           0            0
53            0         0          1           0            0
54            0         0          1           0            0
55            0         0          0           1            0
56            0         0          0           1            0
57            0         0          0           1            0
58            0         0          0           0            1
59            0         0          0           0            1
60            0         0          0           0            1
61            0         0          0           0            0
62            0         0          0           0            0
63            0         0          0           0            0
64            0         0          0           0            0
65            0         0          0           0            0
66            0         0          0           0            0
67            0         0          0           0            0
68            0         0          0           0            0
69            0         0          0           0            0
70            0         0          0           0            0
71            0         0          0           0            0
72            0         0          0           0            0
73            0         0          0           0            0
74            0         0          0           0            0
75            0         0          0           0            0
76            0         0          0           0            0
77            0         0          0           0            0
78            0         0          0           0            0
79            0         0          0           0            0
80            0         0          0           0            0
81            0         0          0           0            0

Make contrasts

contrasts <- makeContrasts(levels=design,
  # Comparing baseline samples with other age groups (49 and 81)
  ## Blood
  d.AL.49.Blood    = AL.49.Blood    - BASELINE.10.Blood,
  d.CCR.49.Blood   = CCR.49.Blood   - BASELINE.10.Blood,
  d.ICRR.49.Blood  = ICRR.49.Blood  - BASELINE.10.Blood,
  d.ICRRF.49.Blood = ICRRF.49.Blood - BASELINE.10.Blood,
  d.AL.81.Blood    = AL.81.Blood    - BASELINE.10.Blood,
  d.CCR.81.Blood   = CCR.81.Blood   - BASELINE.10.Blood,
  d.ICRR.81.Blood  = ICRR.81.Blood  - BASELINE.10.Blood,
  d.ICRRF.81.Blood = ICRRF.81.Blood - BASELINE.10.Blood,
  ## Brain
  d.AL.49.Brain    = AL.49.Brain    - BASELINE.10.Brain,
  d.CCR.49.Brain   = CCR.49.Brain   - BASELINE.10.Brain,
  d.ICRR.49.Brain  = ICRR.49.Brain  - BASELINE.10.Brain,
  d.ICRRF.49.Brain = ICRRF.49.Brain - BASELINE.10.Brain,
  d.AL.81.Brain    = AL.81.Brain    - BASELINE.10.Brain,
  d.CCR.81.Brain   = CCR.81.Brain   - BASELINE.10.Brain,
  d.ICRR.81.Brain  = ICRR.81.Brain  - BASELINE.10.Brain,
  d.ICRRF.81.Brain = ICRRF.81.Brain - BASELINE.10.Brain,
  ## MFP
  d.AL.49.MFP    = AL.49.MFP    - BASELINE.10.MFP,
  d.CCR.49.MFP   = CCR.49.MFP   - BASELINE.10.MFP,
  d.ICRR.49.MFP  = ICRR.49.MFP  - BASELINE.10.MFP,
  d.ICRRF.49.MFP = ICRRF.49.MFP - BASELINE.10.MFP,
  d.AL.81.MFP    = AL.81.MFP    - BASELINE.10.MFP,
  d.CCR.81.MFP   = CCR.81.MFP   - BASELINE.10.MFP,
  d.ICRR.81.MFP  = ICRR.81.MFP  - BASELINE.10.MFP,
  d.ICRRF.81.MFP = ICRRF.81.MFP - BASELINE.10.MFP)

Contrasts

contrasts
                   Contrasts
Levels              d.AL.49.Blood d.CCR.49.Blood d.ICRR.49.Blood d.ICRRF.49.Blood
  BASELINE.10.Blood            -1             -1              -1               -1
  AL.49.Blood                   1              0               0                0
  CCR.49.Blood                  0              1               0                0
  ICRR.49.Blood                 0              0               1                0
  ICRRF.49.Blood                0              0               0                1
  AL.81.Blood                   0              0               0                0
  CCR.81.Blood                  0              0               0                0
  ICRR.81.Blood                 0              0               0                0
  ICRRF.81.Blood                0              0               0                0
  BASELINE.10.Brain             0              0               0                0
  AL.49.Brain                   0              0               0                0
  CCR.49.Brain                  0              0               0                0
  ICRR.49.Brain                 0              0               0                0
  ICRRF.49.Brain                0              0               0                0
  AL.81.Brain                   0              0               0                0
  CCR.81.Brain                  0              0               0                0
  ICRR.81.Brain                 0              0               0                0
  ICRRF.81.Brain                0              0               0                0
  BASELINE.10.MFP               0              0               0                0
  AL.49.MFP                     0              0               0                0
  CCR.49.MFP                    0              0               0                0
  ICRR.49.MFP                   0              0               0                0
  ICRRF.49.MFP                  0              0               0                0
  AL.81.MFP                     0              0               0                0
  CCR.81.MFP                    0              0               0                0
  ICRR.81.MFP                   0              0               0                0
  ICRRF.81.MFP                  0              0               0                0
                   Contrasts
Levels              d.AL.81.Blood d.CCR.81.Blood d.ICRR.81.Blood d.ICRRF.81.Blood
  BASELINE.10.Blood            -1             -1              -1               -1
  AL.49.Blood                   0              0               0                0
  CCR.49.Blood                  0              0               0                0
  ICRR.49.Blood                 0              0               0                0
  ICRRF.49.Blood                0              0               0                0
  AL.81.Blood                   1              0               0                0
  CCR.81.Blood                  0              1               0                0
  ICRR.81.Blood                 0              0               1                0
  ICRRF.81.Blood                0              0               0                1
  BASELINE.10.Brain             0              0               0                0
  AL.49.Brain                   0              0               0                0
  CCR.49.Brain                  0              0               0                0
  ICRR.49.Brain                 0              0               0                0
  ICRRF.49.Brain                0              0               0                0
  AL.81.Brain                   0              0               0                0
  CCR.81.Brain                  0              0               0                0
  ICRR.81.Brain                 0              0               0                0
  ICRRF.81.Brain                0              0               0                0
  BASELINE.10.MFP               0              0               0                0
  AL.49.MFP                     0              0               0                0
  CCR.49.MFP                    0              0               0                0
  ICRR.49.MFP                   0              0               0                0
  ICRRF.49.MFP                  0              0               0                0
  AL.81.MFP                     0              0               0                0
  CCR.81.MFP                    0              0               0                0
  ICRR.81.MFP                   0              0               0                0
  ICRRF.81.MFP                  0              0               0                0
                   Contrasts
Levels              d.AL.49.Brain d.CCR.49.Brain d.ICRR.49.Brain d.ICRRF.49.Brain
  BASELINE.10.Blood             0              0               0                0
  AL.49.Blood                   0              0               0                0
  CCR.49.Blood                  0              0               0                0
  ICRR.49.Blood                 0              0               0                0
  ICRRF.49.Blood                0              0               0                0
  AL.81.Blood                   0              0               0                0
  CCR.81.Blood                  0              0               0                0
  ICRR.81.Blood                 0              0               0                0
  ICRRF.81.Blood                0              0               0                0
  BASELINE.10.Brain            -1             -1              -1               -1
  AL.49.Brain                   1              0               0                0
  CCR.49.Brain                  0              1               0                0
  ICRR.49.Brain                 0              0               1                0
  ICRRF.49.Brain                0              0               0                1
  AL.81.Brain                   0              0               0                0
  CCR.81.Brain                  0              0               0                0
  ICRR.81.Brain                 0              0               0                0
  ICRRF.81.Brain                0              0               0                0
  BASELINE.10.MFP               0              0               0                0
  AL.49.MFP                     0              0               0                0
  CCR.49.MFP                    0              0               0                0
  ICRR.49.MFP                   0              0               0                0
  ICRRF.49.MFP                  0              0               0                0
  AL.81.MFP                     0              0               0                0
  CCR.81.MFP                    0              0               0                0
  ICRR.81.MFP                   0              0               0                0
  ICRRF.81.MFP                  0              0               0                0
                   Contrasts
Levels              d.AL.81.Brain d.CCR.81.Brain d.ICRR.81.Brain d.ICRRF.81.Brain
  BASELINE.10.Blood             0              0               0                0
  AL.49.Blood                   0              0               0                0
  CCR.49.Blood                  0              0               0                0
  ICRR.49.Blood                 0              0               0                0
  ICRRF.49.Blood                0              0               0                0
  AL.81.Blood                   0              0               0                0
  CCR.81.Blood                  0              0               0                0
  ICRR.81.Blood                 0              0               0                0
  ICRRF.81.Blood                0              0               0                0
  BASELINE.10.Brain            -1             -1              -1               -1
  AL.49.Brain                   0              0               0                0
  CCR.49.Brain                  0              0               0                0
  ICRR.49.Brain                 0              0               0                0
  ICRRF.49.Brain                0              0               0                0
  AL.81.Brain                   1              0               0                0
  CCR.81.Brain                  0              1               0                0
  ICRR.81.Brain                 0              0               1                0
  ICRRF.81.Brain                0              0               0                1
  BASELINE.10.MFP               0              0               0                0
  AL.49.MFP                     0              0               0                0
  CCR.49.MFP                    0              0               0                0
  ICRR.49.MFP                   0              0               0                0
  ICRRF.49.MFP                  0              0               0                0
  AL.81.MFP                     0              0               0                0
  CCR.81.MFP                    0              0               0                0
  ICRR.81.MFP                   0              0               0                0
  ICRRF.81.MFP                  0              0               0                0
                   Contrasts
Levels              d.AL.49.MFP d.CCR.49.MFP d.ICRR.49.MFP d.ICRRF.49.MFP
  BASELINE.10.Blood           0            0             0              0
  AL.49.Blood                 0            0             0              0
  CCR.49.Blood                0            0             0              0
  ICRR.49.Blood               0            0             0              0
  ICRRF.49.Blood              0            0             0              0
  AL.81.Blood                 0            0             0              0
  CCR.81.Blood                0            0             0              0
  ICRR.81.Blood               0            0             0              0
  ICRRF.81.Blood              0            0             0              0
  BASELINE.10.Brain           0            0             0              0
  AL.49.Brain                 0            0             0              0
  CCR.49.Brain                0            0             0              0
  ICRR.49.Brain               0            0             0              0
  ICRRF.49.Brain              0            0             0              0
  AL.81.Brain                 0            0             0              0
  CCR.81.Brain                0            0             0              0
  ICRR.81.Brain               0            0             0              0
  ICRRF.81.Brain              0            0             0              0
  BASELINE.10.MFP            -1           -1            -1             -1
  AL.49.MFP                   1            0             0              0
  CCR.49.MFP                  0            1             0              0
  ICRR.49.MFP                 0            0             1              0
  ICRRF.49.MFP                0            0             0              1
  AL.81.MFP                   0            0             0              0
  CCR.81.MFP                  0            0             0              0
  ICRR.81.MFP                 0            0             0              0
  ICRRF.81.MFP                0            0             0              0
                   Contrasts
Levels              d.AL.81.MFP d.CCR.81.MFP d.ICRR.81.MFP d.ICRRF.81.MFP
  BASELINE.10.Blood           0            0             0              0
  AL.49.Blood                 0            0             0              0
  CCR.49.Blood                0            0             0              0
  ICRR.49.Blood               0            0             0              0
  ICRRF.49.Blood              0            0             0              0
  AL.81.Blood                 0            0             0              0
  CCR.81.Blood                0            0             0              0
  ICRR.81.Blood               0            0             0              0
  ICRRF.81.Blood              0            0             0              0
  BASELINE.10.Brain           0            0             0              0
  AL.49.Brain                 0            0             0              0
  CCR.49.Brain                0            0             0              0
  ICRR.49.Brain               0            0             0              0
  ICRRF.49.Brain              0            0             0              0
  AL.81.Brain                 0            0             0              0
  CCR.81.Brain                0            0             0              0
  ICRR.81.Brain               0            0             0              0
  ICRRF.81.Brain              0            0             0              0
  BASELINE.10.MFP            -1           -1            -1             -1
  AL.49.MFP                   0            0             0              0
  CCR.49.MFP                  0            0             0              0
  ICRR.49.MFP                 0            0             0              0
  ICRRF.49.MFP                0            0             0              0
  AL.81.MFP                   1            0             0              0
  CCR.81.MFP                  0            1             0              0
  ICRR.81.MFP                 0            0             1              0
  ICRRF.81.MFP                0            0             0              1

Fitting the new model

We fit the linear model like before

fit <- lmFit(full.data, design)

and now we make a new fit object with the contrasts

fit.cont <- contrasts.fit(fit, contrasts)

then we do the statistical analysis with fir.contr

eb.contr <- eBayes(fit.cont)

## Final result
topTable(eb.contr)
                d.AL.49.Blood d.CCR.49.Blood d.ICRR.49.Blood d.ICRRF.49.Blood
MIMAT0015219_st     -0.011642       0.110148       -0.059414         -0.10279
MIMAT0016984_st     -0.004313       0.008529        0.086223          0.17770
MIMAT0000551_st     -0.123110      -0.348756       -0.078936         -0.17353
MIMAT0001419_st     -0.103899       0.031181       -0.120647         -0.06308
MIMAT0001093_st     -0.095744       0.041754       -0.342382         -0.15005
MIMAT0017081_st     -0.059138       0.155302        0.084894          0.20335
MIMAT0003730_st      0.119957      -0.103752        0.055789         -0.01886
MIMAT0004670_st      0.094842      -0.005905       -0.064414         -0.05073
MIMAT0003169_st      0.030382      -0.013608        0.004325          0.26003
MIMAT0003185_st      0.135141      -0.123001       -0.142064         -0.15755
                d.AL.81.Blood d.CCR.81.Blood d.ICRR.81.Blood d.ICRRF.81.Blood
MIMAT0015219_st      -0.05860        0.17136        -0.06222         0.033516
MIMAT0016984_st       0.25382        0.21633         0.07004         0.061968
MIMAT0000551_st      -0.24694       -0.20006        -0.22763        -0.226651
MIMAT0001419_st      -0.01461       -0.16877         0.03883        -0.115158
MIMAT0001093_st      -0.19678       -0.32657        -0.27456        -0.041006
MIMAT0017081_st       0.16238       -0.11517         0.26811        -0.072199
MIMAT0003730_st      -0.09105        0.01450         0.11660         0.222681
MIMAT0004670_st      -0.08667       -0.07648         0.08011        -0.004351
MIMAT0003169_st       0.14163       -0.01836         0.35665        -0.061431
MIMAT0003185_st      -0.10612       -0.33158        -0.06949        -0.148556
                d.AL.49.Brain d.CCR.49.Brain d.ICRR.49.Brain d.ICRRF.49.Brain
MIMAT0015219_st         1.600          1.628           1.337            1.344
MIMAT0016984_st         1.836          1.842           1.718            1.679
MIMAT0000551_st         1.594          1.654           1.641            1.069
MIMAT0001419_st         2.387          2.224           2.164            1.996
MIMAT0001093_st         2.156          2.223           1.745            1.876
MIMAT0017081_st         2.467          2.325           2.098            1.944
MIMAT0003730_st         2.407          1.866           1.074            1.781
MIMAT0004670_st         2.794          2.713           2.420            2.325
MIMAT0003169_st         2.044          1.841           1.347            1.491
MIMAT0003185_st         2.292          2.309           1.854            2.130
                d.AL.81.Brain d.CCR.81.Brain d.ICRR.81.Brain d.ICRRF.81.Brain
MIMAT0015219_st      -0.70458       -0.85485       -0.451181         -0.62187
MIMAT0016984_st      -0.53646       -1.10695       -1.008464         -1.04613
MIMAT0000551_st      -0.10124       -0.86752       -1.128511         -1.21907
MIMAT0001419_st       0.15496        0.08773       -0.003602         -0.01604
MIMAT0001093_st      -0.13089       -0.70659       -0.273312         -0.65018
MIMAT0017081_st      -0.11468        0.01337       -0.218921         -0.09478
MIMAT0003730_st      -0.30350       -0.41475       -0.443546         -0.89165
MIMAT0004670_st       0.61631       -0.10277       -0.130255         -0.27664
MIMAT0003169_st       0.05450       -0.04489       -0.192080         -0.14153
MIMAT0003185_st      -0.09072       -0.42409       -0.684984         -0.44163
                d.AL.49.MFP d.CCR.49.MFP d.ICRR.49.MFP d.ICRRF.49.MFP d.AL.81.MFP
MIMAT0015219_st  -0.2874612     -0.44967      -0.59178       -0.41424   -0.481127
MIMAT0016984_st   0.0007491      0.11716       0.16021        0.13057    0.350709
MIMAT0000551_st  -0.2825506     -0.23220       0.16451       -0.22562   -0.086961
MIMAT0001419_st   0.0382063      0.05316       0.15798        0.20075    0.186123
MIMAT0001093_st  -0.1859465      0.10607      -0.10746       -0.19937    0.062045
MIMAT0017081_st   0.0206526     -0.04143      -0.11232        0.03181    0.001306
MIMAT0003730_st   0.1237362     -0.01530      -0.02262        0.13350    0.047223
MIMAT0004670_st   0.1162743      0.33883       0.46336        0.22617    0.142874
MIMAT0003169_st   0.0645245      0.02266       0.03574        0.10184    0.080592
MIMAT0003185_st  -0.3047829     -0.28517      -0.23675       -0.01346   -0.026219
                d.CCR.81.MFP d.ICRR.81.MFP d.ICRRF.81.MFP AveExpr     F   P.Value
MIMAT0015219_st    -0.436445       -0.2502      -0.157084  1.5554 21.88 2.167e-20
MIMAT0016984_st     0.139366        0.1909       0.246484  1.5264 18.16 1.855e-18
MIMAT0000551_st    -0.166016       -0.1407      -0.114583  1.1604 15.58 6.539e-17
MIMAT0001419_st     0.078181        0.2905       0.169631  0.9678 15.03 1.504e-16
MIMAT0001093_st     0.071849       -0.1065      -0.208179  1.8640 14.87 1.913e-16
MIMAT0017081_st     0.094600       -0.2538       0.152711  1.2878 13.95 8.121e-16
MIMAT0003730_st     0.517427        0.1752       0.227106  1.1936 12.97 4.099e-15
MIMAT0004670_st    -0.002319        0.1444      -0.008888  1.4753 11.83 3.067e-14
MIMAT0003169_st     0.137778        0.1929       0.193932  1.2518 11.45 6.150e-14
MIMAT0003185_st    -0.314622       -0.2238       0.226443  2.0067 11.05 1.320e-13
                adj.P.Val
MIMAT0015219_st 4.135e-17
MIMAT0016984_st 1.770e-15
MIMAT0000551_st 4.159e-14
MIMAT0001419_st 7.174e-14
MIMAT0001093_st 7.301e-14
MIMAT0017081_st 2.583e-13
MIMAT0003730_st 1.117e-12
MIMAT0004670_st 7.314e-12
MIMAT0003169_st 1.304e-11
MIMAT0003185_st 2.519e-11