Blog of Andrés Aravena
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BioInfo:

Class 6: DNA Sequencing Technologies Overview

October 5, 2018

This class was supposed to be made with student’s presentation. Only one student fulfilled the requirement: Atakan Ayden

Atakan focused on PacBio. The most important ideas of his talk are:

He also showed the following table, which is exactly what we are looking for:

Method Generation Read length (bp) Single pass error rate (%) No. of reads per run Time per run Cost per million bases (USD)
Sanger ABI 3730x1 1st 600-1000 0.001 96 0.5 - 3 h 500
Illumina HiSeq 2500 (Rapid Run) 2nd 2 x 250 0.1 1.2 x 109 (paired) 1 - 6 days 0.04
PacBio RS II: P6-C4 3rd 1.0–1.5 x 104 on average 13 3.5–7.5 x 104 0.5 - 4 h 0.4 - 0.8
Oxford Nanopore MinION 3rd 2–5 x 103 on average 38 1.1–4.7 x 104 50 h 6.44 - 17.9

For more details check the original slides.